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1. DNA ligase
A
Acts as molecular scissors, cutting DNA molecules at specific nucleotide sequences.
B
Joins two DNA fragments together by forming phosphodiester bonds between the two fragments.
C
Is an enzyme involved in protein synthesis.
2. A circular piece of DNA was found to have 6 recognition sites for a particular endonuclease. How many fragments would be produced?
A
5
B
6
C
7
3. Bacteria contain restriction enzymes:
A
to cleave viral DNA or RNA.
B
to produce new plasmids for gene cloning
C
to defend against bacterial infections
4. A restriction enzyme works by
A
cleaving DNA at a specific recognition site
B
breaking phosphodiester bonds between bases
C
restricting DNA so that genes cannot be cloned
5. Difference between sticky and blunt ends is
A
blunt ends do not have overhangs
B
sticky ends can reattach to blunt ends, but blunt ends cannot
C
blunt ends produce fragments of the same length