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1. 
PCR is a laboratory procedure that makes possible the amplification of DNA fragments. If a single molecular of DNA underwent PCR for 5 cycles, how many molecules of DNA would be present at the end of the reaction?
A.
32
B.
25
C.
10
D.
5
2. 
Use this photo of a gel electrophoresis. Use this photo to answer the next 3 questions.
A.
5'-AGTCCCAGAATAAACGGAATTTCGCATGAGAGGGAATGA-3'
B.
5'-TCATTCCCTCTCATGCGAAATTCCGTTTATTCTGGGACT-3'
C.
3'-TCATTCCCTCTCATGCGAAATTCCGTTTATTCTGGGACT-5'
D.
3'-AGTCCCAGAATAAACGGAATTTCGCATGAGAGGGAATGA-5'
3. 
If the original DNA fragment that was sequenced was the CODING strand, the mature mRNA that would be made from this fragment of DNA is:
A.
5'-AGUCCCAGAAUAAACGGAAUUUCGCAUGAGAGGGAAUGA-3'
B.
5'-UCAGGGUCUUAUUUGCCUUAAAGCGUACUCUCCCUUACU-3'
C.
5'-AGUAAGGGAGAGUACGCUUUAAGGCAAAUAAGACCCUGA-3'
D.
5'-UCAUUCCCUCUCAUGCGAAAUUCCGUUUAUUCUGGGACU-3'
4. 
Assume that translation begins with the very first nucleotide of this sequence. If the original DNA fragment that the graduate student was sequencing was the CODING strand, what is the amino acid sequence? Fill in the blanks using single letter amino acid codons Be sure to capitalize your letter.
5. 
Explain why the genetic code is degenerative.