Clearing
Nucleic acids that have negative charges (anionic) use what kind of dye?
Dehydration
Embedding
Trimming
Infiltration
Cationic components such as proteins stain what kind of dye?
Fixation
Basophillic
The paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden
Acidophillic
Small pieces of tissue that are placed in solutions of chemicals that cross-link proteins and inactive degradative enzymes, which PRESERVES CELL AND TISSUE STRUCTURE
The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100%, which REMOVES ALL WATER
Alcohol is removed in organic solvents in which both alcohol and paraffin are alike (miscible)
The tissue is placed in melted paraffin until it becomes completely infiltrated with this substance
The resulting paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome
