Matching Pairs Tissue Prep Steps and Acid DyeOnline version Creative way to know histological terms. by Taylor Shelton 1 Nucleic acids that have negative charges (anionic) use what kind of dye? 2 Trimming 3 Embedding 4 Infiltration 5 Fixation 6 Cationic components such as proteins stain what kind of dye? 7 Clearing 8 Dehydration Acidophillic The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100%, which REMOVES ALL WATER The resulting paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome The paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden The tissue is placed in melted paraffin until it becomes completely infiltrated with this substance Alcohol is removed in organic solvents in which both alcohol and paraffin are alike (miscible) Small pieces of tissue that are placed in solutions of chemicals that cross-link proteins and inactive degradative enzymes, which PRESERVES CELL AND TISSUE STRUCTURE Basophillic
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