Froggy Jumps FixationOnline version Fixation by Marwa Omot 1 Which fixative increases swelling more than any other fixative? a Picric Acid b Acetic Acid c Glyoxal 2 What gives the Bouin solution its distinct yellow color? a HCL b Picric Acid c Acetic Acid 3 Which is used for routine formalin fixation a 10% Zinc Formalin b 10% Aqueous Formalin c 10% Neutral Buffer Formalin 4 This is used to preserve soft and delicate structures a Picric Acid b Osmium Tetroxide c Bouin Solution 5 What is a fixative that creates a gel-like structure that inhibits penetration of subsequent reagents into the tissue? a Non-Coagulant b Coagulant c Non-Additive 6 Which fixative acts like formaldehyde in crosslinking proteins? Its extra aldehyde group is not involved in most crosslinking reactions a Mercuric Chloride b Glutaraldehyde c B-5 7 What type of solution causes the cell to swell and possibly rupture? a Hydrotonic b Hypertonic c Hypotonic 8 A fixative that creates a network of proteins within the tissue that allows solutions to easily penetrate a Non-Coagulant b Additive c Coagulant 9 This fixative is the best at preserving the ultrastructure of a specimen a Glutaraldehyde b Sodium Hydroxide c Osmium Tetroxide 10 Prolonged fixation in 10% Neutral Buffer Formalin for HER2 testing may show a False-Positive results b False-Negative results c Formalin Pigments 11 Which fixative has replaced formaldehyde in some labs because it is less toxic? a Glycogen b Glyoxal c Chromic acid 12 This forms when formaldehyde solutions are exposed to low temperatures. (32°F) a Paraformaldehyde b Acetate Formalin c Alcoholic Formalin 13 Fixative volume needs to be 10 to 12 times greater than the tissue volume a Idk b True c False 14 What are the primary fixatives used for ultrastructural studies? a Aldehydes b Osmium tetroxide c A and B 15 What are specimens postfixed in to preserve lipids a Potassium Dichromate b Osmium Tetroxide c Zinc Salts 16 When should the specimen be placed into a fixative after removal? a After Processing b 90 Minutes c Right Away 17 What is the preferred fixative for carbohydrates? a Gendre Solution b Carnoy Solution c B-5 18 Which solutions must be tracked from cradle to grave ? a Potassium Dichromate b Mercuric Chloride c Both 19 Which fixatives are preferred in preserving nucleic acid? a Acetic Acid b Carnoy solution c Both 20 This is used as both a fixative ingredient and as a stain a Picric Acid b Chromic Acid c Mercuric Chloride 21 Which pigment is preventable but cannot be removed? a Formalin Pigment b Mercury Pigment c Chrome Pigment 22 Which fixatives preserves lipids and makes them insoluble during processing? a Picric Acid b Chromic Acid c 10% NBF 23 Which fixative has a safety hazard of being an explosive? a Sodium hydroxide b Picric Acid c Hollande Solution 24 Which fixative is known for its microanatomical preservation abilities? a Clarke b Helly c Carnoy 25 Which fixative contains copper acetate? a Gendre Solution b Hollande Solution c Bouin Solution 26 Which of the following is NOT a nonaqueous fixative a Carnoy b Clarke c Glyoxal 27 Alcohol 95% saturated with picric acid, Formaldehyde 37-40%, and Glacial acetic acid are ingredients for what fixative? a Bouin Solution b Hollande Solution c Gendre Solution 28 Absolute ethyl alcohol, Chloroform, and Acetic acid, glacial are ingredients for what fixative? a Carnoy b Clarke c Michel 29 What fixative is also known as Buffered picric acid-formaldehye? a Hollande Solution b Zamboni Solution c Carnoy Solution 30 Which of the following is NOT a Picric acid based solution? a Hollande Solution b Orth Solution c Gendre Solution
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