Matching Pairs Histology Chapter 1Online version A fun and creative way to remember histology and its methods. by Taylor Shelton 1 Two Tissue Components 2 cell structures attract what colored dye? 3 staining 4 Sudan black stain 5 what stains sugars? 6 What decreases the concentration of alcohol? 7 conventional light 8 What increases concentration of alcohol? 9 phase contrast 10 Peroxidase 11 rehydration is used to do what? 12 Two types of tissue preparation 13 proteins attract what kind of dye? 14 What does embedding do? 15 Sectioning 16 4 tissue types 17 what does clearing do? 18 Normarski 19 polarizing 20 Calcium ions in calcified bone 21 Alkaline Phosphatase 22 What does the fixation step do? 23 Dehydration does what? 24 Name 5 fixatives 10% Formalin 4% paraformaldehyde 70% Ethanol Methonal Glutaraldehyde and Osmium Tetroxide (EM) Cut tissue into thin slices of about 10 micrometers. Infiltrates tissue with solid medium to facilitate sectioning basic dye with negative charges Dehydration rehydration used to visualize structural features Epithelial Connective Muscular Nervous Cells Extracellular Matrix Paraffin-embedded tissue Frozen tissue In xylene to embed in tissue using alcohol to get out access fluid Remove paraffin PAS- Periodic Acid- Schiff reaction Acid dye with positive charge. It preserves tissue structure
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