Matching Pairs Which microscopic Image fits?Online version A fun way to remember the correct microscopy for viewing tissues. by Taylor Shelton 1 IMAGE of TEM of a kidney cell with acid phosphatase. 2 Definition of Autoradiography 3 Definition of Conventional Light Microscopy 4 IMAGE of fluorescence, uterine cell stain 5 Definition of Electron Microscopy 6 IMAGE of Transmission Electron Microscopy & Autoradiography 7 IMAGE of phase-contrast microscopy 8 IMAGE of polarizing microscopy only showing collagen fibers with intense yellow or orange birefringence. 9 Image of conventional light BRIGHT FIELD microscopy 10 Definition of a Scanning Electron Microscopy 11 Definition of Polarizing Microscopy 12 IMAGE of contrast microscopy 13 Definition of Transmission Electron Microscopy 14 IMAGE of bright-field microscopy, collagen fibers that appear red, with thin elastic fibers with cell nuclei. 15 IMAGE of confocal microscope 16 Definition of Confocal Microscopy 17 Definition of Fluorescence Microscopy 18 Periodic acid-schiff (PAS) 19 Definition of Phase-Contrast Microscopy Gives a greater resolution and is widely used in research used to dissect tissues optically and can also use fluorescence Can NOT see 3D images. uses the interaction of a beam of electrons with a specimen to produce a image 3D type for ex. looking at cells Can see parts of the cell and at a higher resolution Regular light microscope/ bright field Can be used with confocal light and irradiation of tissues using UV light or a laser CAN see 3D images. Electron beam does not pass through the specimen but is scanned across its surface. Reaction stains polysaccharides Used for physics and can rotate the angle of the plane of polarized light Used to see binding receptors and used to localize radioactive material within the tissue
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