The 5' terminus
Elongation
Template strand of DNA
A hairpin loop in the RNA
The coding strand of a strong prokaryotic promoter (differs from idea by only one nucleotide)
Nucleoside triphosphates (NTPs)
Coding strand DNA sequence beginning at the +1 transcription start site: 5'CGTTACGAC...3'
A promoter is ___________ with respect to a gene.
Coding strand of DNA
RNA Polymerase
5'TTGACA.....TATTAT....3' -35.............-10..........
upstream
The multi-subunit enzyme whose sigma subunit recognizes promoter sequences and whose core enzyme builds RNA using the template DNA strand as code.
Transiently pairs with the newly-synthesized RNA / is complimentary to the newly-synthesized RNA.
The end of the RNA molecule that is synthesized and exposed first.
encodes for the following mRNA: 5'CGUUACGAC...3'
This step of transcription occurs after initiation and before termination. It involves the sequential addition of ribonucleotide building blocks to the 3'-OH of the elongating RNA strand.
The building blocks of RNA
The 5'-->3' or "top" strand
can cause termination of transcription by destabilizing the RNA/DNA heteroduplex.
