Matching Pairs K's Test 1Online version Match the following hai. Jitna yaad hai utna karo by KETALI RANA 1 Identify Pathogen.Name the stain used. 2 Identify and name the stain 3 Identify fungus and name the stain used 4 Identify and name the stain 5 Identify Pathogen. Name the stain used. 6 Identify the possible pathogens(Any 4). 7 Identify the parasite. Name the stain used 8 Identify and name the stain 9 Identify and name the stain 10 Identify and name the stain 11 Identify Pathogen. Name the stain used. Gram negative bacilli H pylori Proteus Mycoplasma E coli Aspergillus Flavus LPCB mount Rhizopus LPCB mount Name : Aspergillus Fumigatus Stain : LPCB mount Gram +ve cocci in cluster S aureus Name : Aspergillus Niger Stain : LPCB mount P.falciparum malaria Stain : Geimsa Stain Name : Aspergillus Flavus Stain : LPCB mount Gram Positive Cocci (S aureus in Cluster) Stain: Gram stain Name : Penicillium Stain : LPCB MOUNT ZN stain, Tuberculosis 1 Identify the medium. Probable bacteria ? 2 Identify the medium. Which probable bacteria ? 3 Identify the medium. Describe use of this medium? 4 Identify the medium and write down possible pathogen(any two) 5 Write one example of alfa and beta hemolytic bacteria 6 Identify the medium. Describe use of this medium? 7 Identify medium ? Use ? 8 Identify the test. Write the type of the test. 9 Identify medium ? Use ? 10 Identify the medium. Describe use of this medium? 11 Identify the medium. Write down the type of this medium? Blood Agar Use : Test Hemolytic Property of bacteria TCBS media Use : Isolation of vibrio species. Nutrient Agar( Simple or Basal media ) Use: To Perform Biochemical test, To study colony morphology, pigment demonstration Antimicrobial Susceptibility test Nutrient Agar Use: To Perform Biochemical test, To study colony morphology, pigment demonstration LJ medium Use: To grow Mycobacterium TB MacConkey agar Non Lactose fermenting bacteria Ex: Salmonella , shigella, proteus. alfa : viridans, pnemococci Beta : S. Pyogens , S.aureus Robertson cooked media Use : Anaerobic culture media MaCConkey Agar . Pseudomonas aeruginosa MaCConkey Agar, ex: Ecoli , Klebsiella 1 Identify and Describe use 2 Describe Type, Dose and schedule of vaccine 3 Describe Type, Dose and schedule of vaccine 4 Write down any two use of disinfectant 5 Describe Type, Dose and schedule of vaccine 6 Write down the type of disinfectant and specific use of it 7 Describe Type, Dose and schedule of vaccine 8 Type of disinfectant and write down its uses(any 2) 9 Identify the instrument. Name the use. 10 Identify the instrument. Name the use. 11 Describe Type, Dose and schedule of vaccine 12 Identify the instrument. Name the use. 13 Type of disinfectant and write down the active ingredient Candle jar Use:useful in isolation of capnophilic bacteria.like streptococcus, pneumococcus , gonococcus. Type:Phenolic as antiseptic Active ingredient:Chloroxylenol Type:OPV (live Attenuated Viral) , IPV ( Killed Viral) Dose: OPV:2 drops, IPV: 0.1 ml Schedule :OPV: at Birth, 6 , 10 , 14 weeks. IPV:6,& 14 WEEKS Sterilium (Alcohol & hand rub) Use : To prevent Hospital Acquired infections, before eating food, before and after treating cut or wound Aautoclave ( Horizontal & Vertical) Use: Sterilization of surgical instruments,culture media,autoclavable plastic container,pipette, glassware Type: Phenolic Use: Aa a surface disinfectant in hospital. TYPE : Combined Bacterial Vaccine Dose: 0.5 ml Schedule: Booster 1 : 16-24 months Booster 2: 5-6 years Gaspak system Use: to maintain anaerobic environment and to isolate anaerobic bacteria like pseudomonas TYPE: Viral Combined Dose: 0.5 ml Schedule: 1) 9 complete month, 12 month 2) Booster: 16-24 months Type: Live attenuated bacterial vaccine, Dose: 0.1 ml Schedule: At birth as early as possible. Type: Subunit Viral vaccine Dose : 0.5 ml Schedule: At birth , 6, 10 , 14 weeks along with DPT vaccine Type : Aldehyde Use: preservation of anatomical specimen Formaldehyde gas : fumigation of closed area such as Operation Theater Alcohol Type used as disinfectant and antiseptic , before i.m. or i.v. injection, cleaning of stitches wounds, to clean skin surrounding ulcer 1 Principle and use of test, Name two antigen involved in this test 2 Identify the experimental animal. Write the use of it. 3 Identify the experimental animal. Write the use of it. 4 Identify and give use 5 Identify the biochemical test. Name 2 organism which give positive. 6 Identify the biochemical test. Name 2 organism which give positive. 7 Identify the biochemical test. Name 2 organism which give H2S positive. 8 Identify the experimental animal. Write the use of it. 9 Identify the experimental animal. Write the use of it. 10 Principle and use of test Rabbit Prparation of antisera To see pathogenic effects of bacteria by i.v., i.m. s.c. route To differantiate human & bovine type of tubercul bacilli Sheep 1) To withdraw blood for making blood agar. 2) Sheep RBCs are used in complement fixation test The Carbohydrate Fermentation Test detects an organism's ability to ferment a specific sugar (glucose,maltose) and produce acid,with or without gas. TEST : Citrate Ex: Enterobacter, Klebsiella, Citrobacter Indole Test Ex: Ecoli, proteus , vibrio Cholera Mouse-Foot pad of mice-to maintain Mycobacterium Leprae. Demonstrate ascending and descending type infection of CL.Tetani. Isolate Ricketssia. Agglutination test- H and O antibodies Against S typhi and s paratyphi a and b are detected and measured in patient sera by using O and H antigen Latex Agglutination Test (LAT) These antibodies/antigens react with targets,causing visible clumping. Detect infections,viruses,autoimmune diseases. PROTEUS , SALMONELLA TYPHI , PARATYPHI B Armadillo 1) As a Reservior host of M.leprae 2) To demonstrate drug sensitivity & resistance test.