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eng genètica 3r

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eng genètica 3r

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biotec

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eng genètica 3r
 

eng genètica 3rOnline version

biotec

by Mariona Miquel Garcia
1

Sticky-end ligation is more eûcient than blunt-end ligation

2

It is generally accepted that the optimal length of PCR product is between 18-22 bp

3

The gen called lacZ, encodes for beta-galactosidase, the enzyme involved in the breakdown of X-gal that give a deep blue colored product

4

Linkers are attached to the end of sticky DNA molecule and can be cut to generate blunt ends

5

Klenow fragment can generate a new strand of DNA from an RNA template

6

Cosmids are plasmids with an special module that permits the packaging of recombinant genomes into lambda particles

7

To sequence the entire genome of an organism the most appropriate method is the Sanger method

8

Genomic libraries are constructed using cDNA

9

RT-PCR allows you to compare gene expression between samples

10

The signal peptide that is often included when producing recombinant proteins in Piccia pastors has the role of targeting the recombinant protein to the micocondria where it can accumulate withot tox

11

In RNase protection assay you can only detect one RNA in each reaction

12

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

13

RNA seq uses next-generation sequencing to quantify DNA in a sample at a precise moment in time

14

mRNA is easily puriûed from total RNA using oligodC aûnity columns

15

The substrate for DNA packaging in lambda particles ic circular DNA

16

Lambda can undertake the lysogenic cycle only when the genome is recombinant

17

The yeast two-hybrid system is very useful but it does not allow to identify protein-protein interactions

18

When designing a primer to introduce a change of amino acid in a protein, the mutation or mutations is/are in the center of the mutagenic primer

19

The quick-change mutagenesis kit relies in methylation of the sequence GATC in E.coli and the lack of such modiûcation in newly in vitro-synthesized DNA when using PCR

20

Microarrays can be used to answer this question:

21

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

22

Haripins in primer loop are important for a correct binding to target DNA sequence

23

HIgher number of genes are detected in RNA seq versus Microarrays

24

Different restriction enzymes can generate the same sticky ends

25

In real time PCR a house keeping gene is used as an internal control

26

Northern blot could be used to detect expression of a speciûc gene in diûerent tissues

27

The hybridization probe for primer extension is a synthesized radio labeled oligonucleotide

28

Choosing the appropriate homology arms allows precise integration of DNA sequences in the yeast genome

29

Cell extracts used for in vitro translation provide ribosomes and tRNA

30

.The Gateway cloning technology is based on site-speciûc recombination system

31

In the Northern blot RNA is transferred from the electrophoresis agarose gel to the blotting nitrocellulose membrane

32

In Real Time PCR we always use ûuorescent reporters to follow ampliûcation process

33

An expression cassette consists on a promoter the coding sequences for a particular protein and a transcription termination signal

34

Multiplex-PCR uses one pair of primers annealing to diûerent target sequences

35

Asymmetric PCR uses excess of only one primer and preferentially ampliûes one strand of the target DNA

36

PCR can be used for analyzing genotype or mouse models

37

Depending on your goals or needs, cDNA libraries are prepared in diûerent vectors

38

In RACE PCR you can amplify a full length sequence of an RNA transcript

39

When working with lambda, replacement vectors accept less DNA than the insertion vectors

40

Housekeeping gene has diûerent expression in normal or pathological conditions

41

Polynucleotide kinase dephosphorilates 39-OH ends of DNA

42

When producing a new recombinant protein, independently of your goals, you must always add an aûnity tag

43

In situ hybridization allows you to locate a fragment of nucleic acid whitin a histologic section

44

The northern blot is a technique used to study ene expression by detection of DNA in a sample

45

Random priming is a good method to get speciûc labelling of one of the two DNA strands

46

Oligo(dT) primers are used for cDNA synthesis reactions because of their speciûcity for mRNA

47

All restriction endonucleases recognize hexanucleotide target sites and has a palindromic sequence

48

There exist several technological platforms for high-throughput sequencing. Depending on your needs one platform may be more appropriate than the other

49

In a PCR is always necessary to use degenerate primers

50

Phages derived from f1 that display a fusion protein in their surface cannot be generated so that they contain a genome with the DNA sequence that codes for that particular fusion protein

51

At 94oC, DNA denaturates and strands are separated

52

The Sanger method relies on the Watson and Crick base pairing

53

Polymerases requires a primer to synthese new DNA

54

To identify recombinant colonies, one strategy is to replica plated onto agar medium that contains a second antibiotic, for instance, tetracycline

55

Endonucleases removes nucleotides from the end of a DNA molecule

56

The product of lambda particles containing a cosmic genome requires the lysis of infected cells that contain such genome

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