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Antigen-Antibody Insights -II Quiz

Froggy Jumps

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Challenge your knowledge of immunoassays and antigen–antibody tests.

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Antigen-Antibody Insights -II Quiz
 

Froggy Jumps

Antigen-Antibody Insights -II QuizOnline version

Challenge your knowledge of immunoassays and antigen–antibody tests.

by Quader Ahmed Jalili
1

What does ELISA stand for?

2

Which ELISA type detects antigens in serum using a labeled primary antibody?

3

In Sandwich ELISA, what forms the sandwich?

4

What is the main advantage of ELISA in large labs?

5

Which ELISA variant uses anti-human IgM to capture IgM?

6

What is the principle of competitive ELISA?

7

Which immunoassay is often automated and fluorescence-based?

8

What does CLIA stand for?

9

Which assay uses luminescence detection for higher sensitivity?

10

Western blot is primarily used for what?

11

What does IFA detect?

12

Direct Immunofluorescence Assay uses which component for labelling?

13

Indirect Immunofluorescence Assay detects what?

14

What is ELISA’s main detection readout?

15

ELISPOT is used to quantify which cells?

16

IgG avidity ELISA helps differentiate which infection stage?

17

Which technique uses laser beam for cell analysis, ex: CD4 T cells?

18

What is the main goal of lateral flow immunochromatographic tests?

19

In ICT, what marks the test line at the membrane?

20

What constitutes the control line in ICT rapid tests?

21

What does a positive ELISA indicate in screening?

22

Which assay uses Protein A to detect HIV antibodies among rapid tests?

23

What is a key disadvantage of rapid tests?

24

What is a main advantage of ELISA over rapid tests?

25

Which test confirms ELISA results due to higher specificity?

26

What detection method does IFA primarily use?

27

Which assay is commonly used for Latent TB diagnosis via IFN-γ?

28

What does a ‘sandwich’ ELISA require precoated on the well?

29

Which antigen–antibody technique can yield a color change via chromogen?

30

In MAC ELISA, what is pre-coated on the well?

31

Which method detects multiple antibodies against different antigenic fragments separated by gel electrophoresis?

32

Which assay uses a nitrocellulose membrane for readout?

33

What is a common use of ELISA in infectious diseases?

34

What does a positive HIV TRIDOT flow-through test show?

35

What instrument reads ELISA color changes?

36

Which test is best for differentiating recent from past infections?

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