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Toxoplasmosis: técnicas y medios

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Toxoplasmosis: técnicas y medios

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About this activity

Desafío rápido sobre procesamiento de muestras

Created by

PEDRO GARCÍA LÓPEZ
PEDRO GARCÍA LÓPEZ
Spain

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Toxoplasmosis: técnicas y medios
 

Toxoplasmosis: técnicas y mediosOnline version

Desafío rápido sobre procesamiento de muestras

by PEDRO GARCÍA LÓPEZ
1

El cultivo de T. gondii se realiza en fibroblastos humanos (HFF) usando medio DMEM con 10% FBS.

2

Para muestras de tejidos, la única técnica aceptada es la microscopía de fluorescencia, sin tinciones.

3

Para sangre, se pueden usar serología (IgG/IgM) y PCR para toxoplasmosis.

4

En LCR no se emplea ninguna prueba de detección de T. gondii y solo se usan métodos generales de infección.

5

En tejidos se puede observar T. gondii mediante histología con Giemsa o inmunohistoquímica.

6

La amniocentesis no tiene utilidad alguna en el diagnóstico de toxoplasmosis congénita.

7

Para sangre, el método principal es cultivo directo en agar sangre sin usar técnicas moleculares.

8

El medio de cultivo recomendado para T. gondii es RPMI 1640 con 20% leche y sin FBS.

9

En amnios, se recomienda realizar PCR de amniocentesis para toxoplasmosis congénita.

10

En LCR o líquido cefalorraquídeo se utiliza PCR para detectar Toxoplasma gondii en toxoplasmosis cerebral.

 
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