POLYMERASE CHAIN REACTION NI RYEL

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POLYMERASE CHAIN REACTION: COPYING OF DNDA

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Ryel Monterde

Philippines

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POLYMERASE CHAIN REACTION NI RYEL


Froggy Jumps POLYMERASE CHAIN REACTION NI RYELOnline version POLYMERASE CHAIN REACTION: COPYING OF DNDA by Ryel Monterde 1 A doctor gets no result after PCR testing. Investigation shows the DNA did not separate. What is the most likely problem? a Too many nucleotides b Low temperature during denaturation c Missing primers 2 A forensic scientist wants accurate DNA identification. Why is PCR necessary before analysis? a It increases DNA quantity b It changes DNA structure c It destroys unwanted DNA 3 A student claims PCR can replace the entire central dogma. What is the best evaluation? a Correct, PCR makes proteins b Incorrect, PCR replaces RNA c Incorrect, PCR only copies DNA 4 In an experiment, primers attach to the wrong DNA region. What is the most likely outcome? a Correct DNA is amplified b No DNA is formed c Wrong DNA segment is copied 5 A lab technician forgot to lower the temperature after denaturation. What will happen next? a DNA polymerase works faster b Primers cannot attach c DNA doubles rapidly 6 A researcher wants faster DNA amplification. What is the best strategy? a Reduce cycles b Increase number of cycles c Remove primers 7 During PCR, DNA polymerase stops working. What is the most logical effect? a DNA separates normally b Primers attach correctly c No new DNA strands are formed 8 A sample produces only a small amount of DNA after many cycles. What is a possible reason? a Proper temperature control b Incorrect primer binding c Enough nucleotides 9 Why is PCR considered similar but not identical to DNA replication? a PCR occurs inside the cell b PCR copies RNA c PCR happens outside the cell 10 A scientist wants to detect a virus early. Why is PCR effective? a It removes viruses b It copies even small DNA amounts c It changes viral DNA 11 If nucleotides are lacking in PCR, what will happen? a DNA cannot be built b DNA separates faster c Primers attach quickly 12 A student mixes up the order of PCR steps. What is the most likely result? a Faster amplification b No proper DNA copying c Stronger DNA 13 Why is temperature change essential in PCR? a It colors DNA b It controls each step of the process c It removes DNA 14 A scientist skips repeating PCR cycles. What is the main consequence? a DNA becomes stronger b DNA is not amplified enough c DNA changes structure 15 In PCR, why must primers be specific? a To destroy DNA b To ensure correct DNA is copied c To increase temperature 16 A medical test gives a false result due to contamination. What PCR issue caused this? a Extra unwanted DNA was amplified b DNA was destroyed c No primers were added 17 Why is PCR powerful in forensic investigations? a It changes DNA identity b It copies DNA from very small samples c It removes DNA evidence 18 A student says more cycles always mean better results. What is the best analysis? a Always correct b Always wrong c Too many cycles can cause errors 19 If annealing temperature is too high, what will happen? a DNA separates b DNA separates c DNA doubles faster 20 How does PCR support understanding of genetics? a By removing genes b By copying DNA for study c By changing traits

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POLYMERASE CHAIN REACTION NI RYEL

Froggy Jumps

About this activity

Created by

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POLYMERASE CHAIN REACTION NI RYELOnline version

by Ryel Monterde

A doctor gets no result after PCR testing. Investigation shows the DNA did not separate. What is the most likely problem?

A forensic scientist wants accurate DNA identification. Why is PCR necessary before analysis?

A student claims PCR can replace the entire central dogma. What is the best evaluation?

In an experiment, primers attach to the wrong DNA region. What is the most likely outcome?

A lab technician forgot to lower the temperature after denaturation. What will happen next?

A researcher wants faster DNA amplification. What is the best strategy?

During PCR, DNA polymerase stops working. What is the most logical effect?

A sample produces only a small amount of DNA after many cycles. What is a possible reason?

Why is PCR considered similar but not identical to DNA replication?

A scientist wants to detect a virus early. Why is PCR effective?

If nucleotides are lacking in PCR, what will happen?

A student mixes up the order of PCR steps. What is the most likely result?

Why is temperature change essential in PCR?

A scientist skips repeating PCR cycles. What is the main consequence?

In PCR, why must primers be specific?

A medical test gives a false result due to contamination. What PCR issue caused this?

Why is PCR powerful in forensic investigations?

A student says more cycles always mean better results. What is the best analysis?

If annealing temperature is too high, what will happen?

How does PCR support understanding of genetics?