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PCR and Gel Electrophoresis Quiz

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PCR & gel basics

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PCR and Gel Electrophoresis Quiz
 

PCR and Gel Electrophoresis QuizOnline version

PCR & gel basics

by Selvabharathi Tn
1

Gel electrophoresis uses magnetic fields to move DNA fragments.

2

Taq polymerase extends new DNA strands during the extension step.

3

PCR requires primers that flank the target sequence.

4

Gel electrophoresis separates DNA fragments by size in an agarose matrix.

5

PCR amplifies a target DNA region exponentially through repeated cycles.

6

A DNA ladder in gel is used to stabilize the gel matrix.

7

Ethidium bromide or SYBR Safe stains visualize DNA bands under UV or blue light.

8

The optimal temperature for Taq polymerase is around 60°C.

9

PCR can amplify RNA directly without reverse transcription.

10

DNA polymerase creates cuts at restriction sites during PCR.

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