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Froggy Jumps
Froggy Jumps

Cuestionario de Extracción de RNA y qPCR

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Cuestionario de Extracción de RNA y qPCR

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Cuestionario de Extracción de RNA y qPCR

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Cuestionario de Extracción de RNA y qPCR
 

Froggy Jumps

Cuestionario de Extracción de RNA y qPCROnline version

Cuestionario de Extracción de RNA y qPCR

by :)
1

¿Qué indica la presencia de dos bandas correspondientes a los rRNA 28S y 18S en un gel de agarosa?

2

En la síntesis de cDNA, ¿cuál es la función de la transcriptasa reversa?

3

¿En qué fase de la curva de amplificación de qPCR se realiza la cuantificación de la muestra?

4

El valor Ct (Threshold Cycle) en un experimento de qPCR se define como:

5

​Si necesitas obtener 500 ng de ARN y tu muestra tiene una concentración de 100 ng/µL, ¿qué volumen de ARN debes usar?

6

​Al preparar el Master Mix para 4 muestras, ¿por qué el protocolo sugiere prepararlo para 5 reacciones?

7

​¿Cuál es la utilidad técnica de la incubación final a 4ºC en la síntesis de cDNA?

8

​¿Por qué es fundamental que los primers no formen "dímeros" en qPCR con SYBR Green?

9

​¿Cuál es el propósito del paso de "Enzyme Activation" (95ºC por 3 min) en qPCR?

10

​¿Qué indica observar dos picos de temperatura distintos en la curva de melting?

11

​Si el NTC (No Template Control/Blanco) muestra una curva de amplificación, ¿qué indica?

12

Si tras la extracción de ARN obtienes una relación A260/A230 de 1.2, ¿qué problema metodológico es más probable que haya ocurrido?

 
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