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Froggy Jumps
Froggy Jumps

Detector Fluorescencia HPLC

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Detector Fluorescencia HPLC

Froggy Jumps

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Detector Fluorescencia HPLC

Created by

Mariana Lopez
Mariana Lopez
Colombia

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Detector Fluorescencia HPLC
 

Froggy Jumps

Detector Fluorescencia HPLCOnline version

Detector Fluorescencia HPLC

by Mariana Lopez
1

En comparación con un detector UV, el detector de fluorescencia es más selectivo porque:

2

¿Por qué la derivatización puede mejorar la sensibilidad?

3

¿Qué característica tienen los compuestos del segundo articulo (se usó OPA)?

4

¿Qué tipo de señal se obtiene en el detector de fluorescencia en HPLC?

5

¿Qué relación existe entre el área bajo el pico y la concentración del analito?

6

¿Cuál es un ejemplo de limitación del detector de fluorescencia?

7

¿Qué reactivo se menciona para derivatizar aminas primarias?

8

Si un analito no presenta fluorescencia natural y no se realiza derivatización, ¿qué resultado se esperaría en el cromatograma?

9

¿Cuál sería la consecuencia de una derivatización incompleta del analito?

 
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