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Froggy Jumps
Froggy Jumps

PCR y PCR transcriptasa reversa

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PCR y PCR transcriptasa reversa

Froggy Jumps

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About this activity

Vamos a ver qué tanto aprendieron de estas fabulosas técnicas, los retamos a contestar estas preguntas muy sencillas

Created by

Sergio Ernesto Avila Morales
Sergio Ernesto Avila Morales
Mexico

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PCR y PCR transcriptasa reversa
 

Froggy Jumps

PCR y PCR transcriptasa reversaOnline version

Vamos a ver qué tanto aprendieron de estas fabulosas técnicas, los retamos a contestar estas preguntas muy sencillas

by Sergio Ernesto Avila Morales
1

¿Cuál es el objetivo fundamental de la Reacción en Cadena de la Polimerasa?

2

¿Cuál es el orden lógico de un ciclo de PCR para que la síntesis sea exitosa?

3

¿Por qué se utiliza la Taq polimerasa de la bacteria Thermus aquaticus en lugar de una polimerasa humana?

4

A nivel químico, ¿qué sucede exactamente durante la etapa de desnaturalización?

5

Si la eficiencia es del 100%, ¿cuántas copias de ADN de doble cadena tendrás al finalizar el 4to ciclo si empezaste con una sola?

6

¿Qué componente de la mezcla actúa como el "GPS" para delimitar la región exacta del ADN que queremos copiar?

7

¿Cuál es el molde inicial que utilizamos en una RT-PCR?

8

¿De dónde se aisló originalmente la enzima Transcriptasa inversa?

9

¿Cuál es la principal ventaja de la RT-PCR de dos pasos?

10

¿Para qué se realiza el calentamiemto a 70°C antes de la retrotranscripción?

11

¿Qué información biológica obtenemos fundamentalmente al realizar el RT-PCR?

 
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