Glycolysis Master Quiz

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Advanced glycolysis mastery challenge

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Glycolysis Master Quiz


Froggy Jumps Glycolysis Master QuizOnline version Advanced glycolysis mastery challenge by Student 1 Where do enzymes required for glycolysis primarily reside? a Nucleus b Cytosol c Mitochondrial matrix 2 Glycolysis is a catabolic pathway yielding which energy currency? a NADH b GTP c ATP 3 Can glycolysis function without oxygen? a Only with mitochondria b No c Yes 4 Which substrate is the main fuel for the brain mentioned in the slides? a Glucose b Amino acids c Fatty acids 5 Mature erythrocytes lack which organelle to avoid using their oxygen? a Mitochondria b Ribosomes c Nucleus 6 What is the initial committed step of glycolysis? a Pyruvate kinase b Phosphofructokinase-1 (PFK-1) c Hexokinase 7 Which enzyme is inhibited by its product in glycolysis? a Hexokinase b Pyruvate kinase c Phosphofructokinase-1 8 Which isozyme of hexokinase is expressed mainly in the liver? a Hexokinase II b Hexokinase I c Glucokinase (hexokinase IV) 9 What happens to excess glucose-6-phosphate in the liver after a meal? a Excreted in urine b Used for glycogen synthesis and lipogenesis c Degraded to fat immediately 10 Which enzyme dephosphorylates glucose-6-phosphate in the liver? a Glucone-6-phosphate synthase b Hexokinase c Glucose-6-phosphatase 11 What is the fate of DHAP in glycolysis? a Degraded to acetyl-CoA b Isomerized to glyceraldehyde-3-phosphate c Converted to lactate 12 What is the first oxidation-reduction step in glycolysis? a Fructose-1,6-bisphosphate to DHAP b Glyceraldehyde-3-phosphate to 1,3-BPG c Glyceraldehyde to glyceraldehyde-3-phosphate 13 Which molecule donates phosphate to form ATP in glycolysis? a PEP b 1,3-BPG c HDG 14 Which step yields ATP via substrate-level phosphorylation in glycolysis? a DHAP to glyceraldehyde-3-P b 1,3-BPG to 3-PG c Glucose to glucose-6-P 15 What molecule shifts a phosphate during step 8 of glycolysis? a Enolase b Aldolase c Phosphoglycerate mutase 16 What intermediate is formed just before pyruvate in glycolysis? a 3-Phosphoglycerate b Phosphoenolpyruvate (PEP) c 2-Phosphoglycerate 17 Which enzyme catalyzes the final glycolytic step to pyruvate? a Hexokinase b Pyruvate kinase c Phosphofructokinase-1 18 What is the fate of pyruvate under anaerobic conditions in muscle? a Shuttled to mitochondria b Oxidized to acetyl-CoA c Reduced to lactate 19 NADH produced in glycolysis must be reoxidized by which process? a Beta-oxidation b Glycogenolysis c ETC or lactate formation 20 Which shuttle keeps glycolysis running when oxygen is absent? a Lactate dehydrogenase pathway b Malate-aspartate shuttle c Glyoxylate cycle 21 Which enzyme is a key allosteric regulator of glycolysis? a Phosphofructokinase-1 (PFK-1) b Aldolase c Mutase 22 What is the most potent activator of PFK-1? a ATP b Citrate c Fructose-2,6-bisphosphate 23 Which glycolytic step is irreversible and rate-limiting? a Glyceraldehyde-3-phosphate dehydrogenase step b PFK-1 step (Fructose-6-phosphate to Fructose-1,6-bisphosphate) c Hexokinase step 24 What is the fate of two pyruvate molecules from one glucose in anaerobic glycolysis? a Two ethanol and two ATP b Two acetyl-CoA and two CO2 c Two lactate and two ATP 25 Which molecule is the carrier for electrons during glycolysis? a FAD/FADH2 b NAD+/NADH c CoA-SH 26 Which organism is noted as having low glycolytic activity under ischemia? a Liver b Skeletal muscle c Cardiac muscle 27 Is glucose the principal fuel of the brain according to the slides? a Yes b Only during fasting c No 28 What happens to glucose-6-phosphate when transport into the liver is high? a Converted to glucose-6-phosphate for oxidation or storage b Exits to blood c Degraded to fat immediately 29 Which molecule is used to remake ATP from ADP during glycolysis? a ADP synthase b High-energy phosphate from 1,3-BPG c Glucose-6-phosphate 30 What is liberated in the glycolytic pathway as phosphate groups are transferred? a NADH b ATP c FADH2 31 Which enzyme acts as a key regulator and is inducible? a PFK-1 b Hexokinase c Glyceraldehyde-3-phosphate dehydrogenase 32 What does 2,3-bisphosphoglycerate relate to in glycolysis? a Glycogen synthesis b Allosteric regulation of PFK-1 c Glycerol metabolism 33 Which molecule is the first substrate for glycolysis? a Glucose b Fructose c Galactose 34 What is the role of hexokinase in glycolysis? a Produces ATP b Isomerizes glucose-6-P c Phosphorylates glucose to glucose-6-phosphate 35 Where is glucokinase mainly active? a Liver b Brain c Muscle 36 What happens to pyruvate after glycolysis under aerobic conditions? a Excreted as lactate b Converted to acetyl-CoA c Reduced to lactate 37 Which step is associated with substrate-level phosphorylation producing ATP in glycolysis? a Phosphoglycerate kinase step b Isomerase step c Enolase step 38 Which stage follows glycolysis to complete glucose oxidation? a TCA cycle (Krebs) b Pentose phosphate pathway c Glycogenolysis 39 Which molecule is primarily produced from glucose in aerobic glycolysis? a GTP and NADPH b ATP and NADH c FADH2 and CO2 40 Which enzyme produces dihydroxyacetone phosphate from fructose-1,6-bisphosphate? a Phosphoglucose isomerase b Aldolase c Triose phosphate isomerase 41 What does triose phosphate isomerase do? a Oxidizes glyceraldehyde-3-P b Phosphorylates DHAP c Interconverts DHAP and glyceraldehyde-3-phosphate 42 Which molecule is used to regenerate NAD+ in anaerobic glycolysis? a FADH2 b NADP+ c Lactate from pyruvate via LDH 43 What is the net ATP yield of glycolysis per glucose in anaerobic conditions? a 2 ATP b 0 ATP c 4 ATP 44 What is the main purpose of glycolysis in red blood cells? a Provide ATP since they lack mitochondria b Synthesize glucose c Produce lactate for export 45 What catalyzes the conversion of PEP to pyruvate? a Enolase b Pyruvate kinase c Phosphoglycerate mutase 46 Which glycolytic intermediate donates a phosphate to ADP to form ATP? a 1,3-BPG b 2-PG c 3-PG 47 Which glycolytic enzyme is inhibited by glucose-6-phosphate? a Hexokinase b PFK-1 c Glucokinase 48 Which step is considered the glycolysis committed step? a Pyruvate kinase step b PFK-1 catalyzed step c Hexokinase step 49 Which molecule carries the high-energy phosphate for ATP synthesis in the payoff phase? a 1,3-BPG b 2-PG c 3-PG 50 What is the overall stoichiometry of glycolysis from glucose to lactate? a Glucose + 2 ADP + 2 Pi -> 2 Lactate + 2 ATP + 2 H2O b Glucose + 2 NAD+ -> 2 Pyruvate + 2 NADH 51 Which cellular condition favors anaerobic glycolysis in muscles? a Low oxygen availability b High oxygen c High CO2 52 Which statement about glycolysis is true? a It can function aerobically or anaerobically b It only functions anaerobically c It requires mitochondria 53 Which enzyme is a key regulatory control point in glycolysis? a Aldolase b PFK-1 c Hexokinase 54 What does the glycolytic pathway produce besides ATP? a Metabolic intermediates b CO2 only c Oxygen 55 Which option best describes glycolysis location in erythrocytes? a Nucleus b Cytosol c Mitochondria 56 What is the fate of lactate produced in anaerobic glycolysis? a Reoxidation to pyruvate in liver or Cori cycle b Conversion to acetyl-CoA in muscle c Excretion in urine 57 Which molecule is not present in mature erythrocytes but necessary for glycolysis? a Ribosomes b Mitochondria c Nucleus 58 What happens to NAD+ during glycolysis when oxygen is scarce? a Converted to NADP+ b Degraded to NADH c Reoxidized by lactate formation or ETC when possible 59 Which molecule indicates glycolysis’ integration with lipid synthesis in liver? a Acetyl-CoA b Glucose-6-phosphate c Citrate 60 Which glycolytic enzyme is bound to the outer mitochondrial membrane in muscle? a Hexokinase b Glucokinase c PFK-1 61 What is the role of glyceraldehyde-3-phosphate dehydrogenase in glycolysis? a Oxidizes G3P and reduces NAD+ to NADH b Isomerizes DHAP c Phosphorylates G3P 62 What is the end product of glycolysis under aerobic conditions in most cells? a Pyruvate b Acetyl-CoA c Lactate 63 Which step in glycolysis yields NADH? a Enolase step b Phosphoglycerate kinase step c Glyceraldehyde-3-phosphate dehydrogenase step 64 What is the net production of ATP per glucose under aerobic glycolysis including the payoff phase? a 32 ATP only from glycolysis b 2 ATP from glycolysis plus others in TCA/ETC c 0 ATP 65 What is the key function of hexokinase in glycolysis? a Transports glucose into mitochondria b Phosphorylates glucose to trap it in the cell c Removes phosphate from glucose-6-phosphate 66 Which molecule acts as the allosteric activator of PFK-1? a Fructose-2,6-bisphosphate b ATP c Citrate 67 In the fasted state, where is glucokinase held? a In the cytosol b On the outer mitochondrial membrane c In the nucleus bound to regulatory protein 68 Which pathway continues glucose oxidation after glycolysis in the presence of oxygen? a Pentose phosphate pathway b Citric acid cycle (Krebs) c Gluconeogenesis 69 What molecule shifts in glycolysis to create phosphoglycerate? a Methyl shift to 4-PG b Hydration to 3-PG c Phosphate group from 3-PG to 2-PG 70 Which enzyme regenerates NAD+ in anaerobic glycolysis? a Isocitrate dehydrogenase b Malate dehydrogenase c Lactate dehydrogenase 71 Which molecule is a substrate-level phosphoryl donor in glycolysis? a NADH b FADH2 c 1,3-BPG 72 Which statement best describes glycolysis’ net energy yield? a Net ATP gain with energy intermediates b Net ATP loss c Net NADH loss 73 What is the main energy product of glycolysis in erythrocytes? a FADH2 b NADH c ATP 74 Which step forms dihydroxyacetone phosphate (DHAP)? a Aldolase cleavage of fructose-1,6-bisphosphate b Phosphofructokinase-1 step c Triose phosphate isomerase step 75 Which glycolytic enzyme is directly inhibited by its product? a Hexokinase b Pyruvate kinase c Glucokinase 76 Which molecule marks the entry of glucose into glycolysis? a Glucose-6-phosphate b Glucose-1-phosphate c Fructose-1,6-bisphosphate 77 What is the fate of lactate in liver? a Excreted in urine b Stored as fat c Converted to glucose via gluconeogenesis 78 What is the energy investment phase of glycolysis? a ATP-generating steps only b No energy input c Initial ATP-consuming steps before payoff 79 Which major energy pathway is linked to glycolysis via acetyl-CoA production? a Citric acid cycle (Krebs) b Pentose phosphate pathway c Gluconeogenesis 80 What cofactor is reduced during glyceraldehyde-3-phosphate oxidation? a CoA-SH b NAD+ to NADH c FAD to FADH2 81 Which molecule is the final electron acceptor in aerobic glycolysis? a NAD+ b Oxygen c Pyruvate

Glycolysis Master Quiz

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Glycolysis Master QuizOnline version

by Student

Where do enzymes required for glycolysis primarily reside?

Glycolysis is a catabolic pathway yielding which energy currency?

Can glycolysis function without oxygen?

Which substrate is the main fuel for the brain mentioned in the slides?

Mature erythrocytes lack which organelle to avoid using their oxygen?

What is the initial committed step of glycolysis?

Which enzyme is inhibited by its product in glycolysis?

Which isozyme of hexokinase is expressed mainly in the liver?

What happens to excess glucose-6-phosphate in the liver after a meal?

Which enzyme dephosphorylates glucose-6-phosphate in the liver?

What is the fate of DHAP in glycolysis?

What is the first oxidation-reduction step in glycolysis?

Which molecule donates phosphate to form ATP in glycolysis?

Which step yields ATP via substrate-level phosphorylation in glycolysis?

What molecule shifts a phosphate during step 8 of glycolysis?

What intermediate is formed just before pyruvate in glycolysis?

Which enzyme catalyzes the final glycolytic step to pyruvate?

What is the fate of pyruvate under anaerobic conditions in muscle?

NADH produced in glycolysis must be reoxidized by which process?

Which shuttle keeps glycolysis running when oxygen is absent?

Which enzyme is a key allosteric regulator of glycolysis?

What is the most potent activator of PFK-1?

Which glycolytic step is irreversible and rate-limiting?

What is the fate of two pyruvate molecules from one glucose in anaerobic glycolysis?

Which molecule is the carrier for electrons during glycolysis?

Which organism is noted as having low glycolytic activity under ischemia?

Is glucose the principal fuel of the brain according to the slides?

What happens to glucose-6-phosphate when transport into the liver is high?

Which molecule is used to remake ATP from ADP during glycolysis?

What is liberated in the glycolytic pathway as phosphate groups are transferred?

Which enzyme acts as a key regulator and is inducible?

What does 2,3-bisphosphoglycerate relate to in glycolysis?

Which molecule is the first substrate for glycolysis?

What is the role of hexokinase in glycolysis?

Where is glucokinase mainly active?

What happens to pyruvate after glycolysis under aerobic conditions?

Which step is associated with substrate-level phosphorylation producing ATP in glycolysis?

Which stage follows glycolysis to complete glucose oxidation?

Which molecule is primarily produced from glucose in aerobic glycolysis?

Which enzyme produces dihydroxyacetone phosphate from fructose-1,6-bisphosphate?

What does triose phosphate isomerase do?

Which molecule is used to regenerate NAD+ in anaerobic glycolysis?

What is the net ATP yield of glycolysis per glucose in anaerobic conditions?

What is the main purpose of glycolysis in red blood cells?

What catalyzes the conversion of PEP to pyruvate?

Which glycolytic intermediate donates a phosphate to ADP to form ATP?

Which glycolytic enzyme is inhibited by glucose-6-phosphate?

Which step is considered the glycolysis committed step?

Which molecule carries the high-energy phosphate for ATP synthesis in the payoff phase?

What is the overall stoichiometry of glycolysis from glucose to lactate?

Which cellular condition favors anaerobic glycolysis in muscles?

Which statement about glycolysis is true?

Which enzyme is a key regulatory control point in glycolysis?

What does the glycolytic pathway produce besides ATP?

Which option best describes glycolysis location in erythrocytes?

What is the fate of lactate produced in anaerobic glycolysis?

Which molecule is not present in mature erythrocytes but necessary for glycolysis?

What happens to NAD+ during glycolysis when oxygen is scarce?

Which molecule indicates glycolysis’ integration with lipid synthesis in liver?

Which glycolytic enzyme is bound to the outer mitochondrial membrane in muscle?

What is the role of glyceraldehyde-3-phosphate dehydrogenase in glycolysis?