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Stages in Translation

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Stages in Translation

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Overview of translation stages

Created by

aqidah
aqidah
Malaysia

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Stages in TranslationOnline version

Overview of translation stages

by aqidah
1

The stages involved in translation : Stage 1 : Stage 2 : Stage 3 :

2

Small ribosomal subunit with tRNA binds at 5 ? cap of mRNA And then move along the mRNA until reaches codon . tRNA which carries amino acid ( with anticodon ( 3 ? - - 5 ? ) reach to the start codon ( 5 ? - - 3 ? ) at mRNA by forming bonds . Large ribosomal subunit attaches with these complex to form translation initiation . Initiator tRNA is now located in the site of the ribosome , site is empty

3

Consists of a series of three step cycles as each amino acid is added one by one to the C - terminus of the growing chain . Stage 2 : a ) recognition b ) Peptide bond formation c )

4

During codon recognition , - tRNA with anticodon which is complementary to the mRNA enters to the empty site

5

Peptide bond is formed between amino acid in the site & the new amino acid in site . Catalyzes by . This step removes the growing from the tRNA in the P site and binds it to the new amino acid on the tRNA in the site by a bon d

6

During , the ribosome moves one codon ahead ( from 5 ? to 3 ? ) . tRNA in the site is moved to the site & then leaves the ribosome . The ( with the attached polypeptide ) in the site is translocated to the P sit e

7

The next codon is now available at the site . The three steps of elongation continue by codon to add amino acids until the chain is completed .

8

occurs when the site of the ribosome reaches one of the three codons ( 5 ? - UAG - 3 ? / 5 ? - UAA - 3 ? / 5 ? - - 3 ? ) on mRNA . The stop codon does not code for amino acids ; thus no tRNA will bind to the stop codon . A binds directly to the stop codon in the A site . It causes the addition of water molecules , the bond between the polypeptide and the tRNA in the P site . Newly synthesized protein is released . Translation complex dissociates with the hydrolysis of GTP molecules .

 
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