Glycolysis Quiz ChallengeOnline version Test your knowledge on the first five steps of glycolysis with this fun quiz! by Tola Alabi 1 What is the first enzyme involved in glycolysis? a Hexokinase b Aldolase c Phosphofructokinase d Enolase 2 What is the product of the first step in glycolysis? a Fructose-1,6-bisphosphate b Dihydroxyacetone phosphate c Glucose-6-phosphate d Pyruvate 3 Which enzyme catalyzes the conversion of glucose to glucose-6-phosphate? a Phosphoglycerate kinase b Hexokinase c Triose phosphate isomerase d Aldolase 4 What is the second step of glycolysis? a Isomerization of glucose-6-phosphate b Oxidation of glyceraldehyde-3-phosphate c Phosphorylation of glucose d Cleavage of fructose-1,6-bisphosphate 5 Which enzyme is responsible for converting glucose-6-phosphate to fructose-6-phosphate? a Phosphoglucose isomerase b Hexokinase c Aldolase d Phosphofructokinase 6 What is the product of the third step in glycolysis? a Fructose-1,6-bisphosphate b Glucose-6-phosphate c Dihydroxyacetone phosphate d Glyceraldehyde-3-phosphate 7 Which enzyme catalyzes the phosphorylation of fructose-6-phosphate? a Hexokinase b Phosphofructokinase c Aldolase d Enolase 8 What is the fourth step of glycolysis? a Formation of pyruvate b Oxidation of glyceraldehyde-3-phosphate c Cleavage of fructose-1,6-bisphosphate d Isomerization of glucose-6-phosphate 9 What are the two products formed from the cleavage of fructose-1,6-bisphosphate? a Acetyl-CoA and NADH b Fructose-6-phosphate and pyruvate c Glucose and glucose-6-phosphate d Glyceraldehyde-3-phosphate and dihydroxyacetone phosphate 10 Which enzyme converts dihydroxyacetone phosphate into glyceraldehyde-3-phosphate? a Enolase b Triose phosphate isomerase c Aldolase d Phosphofructokinase