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Pistas sobre Inmunofluorescencia

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Pistas sobre Inmunofluorescencia

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About this activity

Técnica que ilumina proteínas.

Created by

JOSE IVAN PATRACA FIERRO
JOSE IVAN PATRACA FIERRO
Mexico

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Pistas sobre Inmunofluorescencia
 

Pistas sobre InmunofluorescenciaOnline version

Técnica que ilumina proteínas.

by JOSE IVAN PATRACA FIERRO
1

Revela proteínas en muestras gracias a anticuerpos coloreados.

Pistas

Existen versiones directa e indirecta para localizar antígenos en células.

Permite observar bajo un microscopio de fluorescencia.

Se usan anticuerpos etiquetados con fluoróforos para detectar antígenos.

2

¿Cuál es la principal ventaja física de utilizar el método indirecto (anticuerpo primario + secundario) sobre el método directo?

Pistas

C R G N C

3

Estás capturando una imagen de fluorescencia y notas que el brillo disminuye rápidamente mientras observas la muestra. ¿Cómo se llama este proceso y cómo lo mitigarías?

Pistas

¿la vitamina C es un?

4

Al analizar la co-localización de dos canales (rojo y verde) en Fiji, obtienes un coeficiente de Pearson de 0.05. ¿Qué significa biológicamente?

Pistas

solapamiento

5

En un protocolo de IF, el paso de 'Recuperación de Antígenos' (HIER) tiene como objetivo:

Pistas

los puentes de metileno se forman por el formaldehido en la fijacion

6

En un experimento de inmunofluorescencia, observas que la luz de emisión tiene una longitud de onda mayor que la de excitación. ¿Qué principio físico explica esta pérdida de energía?

Pistas

Desplazamiento

7

¿Cuál es la función del 'Pinhole' en un microscopio confocal al realizar inmunofluorescencia?

Pistas

fuera del plano focal o luz de sobra

 
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