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HIBRIDACIÓN TEST

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HIBRIDACIÓN TEST

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Hibridación

Created by

adriana
adriana
Ecuador

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HIBRIDACIÓN TEST
 

HIBRIDACIÓN TESTOnline version

Hibridación

by adriana
1

Unión de dos cadenas complementarias de ácidos nucleicos (ADN o ARN) mediante la interacción de sus bases nitrogenadas, formando una molécula bicatenaria. ​

2

Biomoléculas que almacenan y transmiten información genética.

3

Cadena de nucleótidos marcada que es complementaria a una secuencia diana y se utiliza para identificar y localizar dicha secuencia. ​

4

Fragmento específico de bases nitrogenadas en el ADN o ARN que se desea detectar mediante hibridación.

5

Procedimiento para marcar las sondas utilizadas en las técnicas de hibridación con el fin de visualizar los resultados.

6

Elementos químicos que emiten radiaciones y se utilizan para marcar sondas en técnicas de hibridación. ​ Ejemplos: Fósforo-32 (³²P) y Tritio (³H). ​

7

Compuestos químicos que emiten luz al ser excitados por luz ultravioleta. ​ Se utilizan para marcar sondas en técnicas como FISH. ​

8

Moléculas pequeñas que se acoplan a nucleótidos sin alterar la especificidad de la sonda. ​ Requieren métodos indirectos de revelado, como inmunológicos o químicos.

9

Técnica de hibridación en soporte sólido que permite analizar múltiples muestras simultáneamente al colocar gotas de muestra sobre una membrana.​

10

Técnica de hibridación en soporte sólido que detecta fragmentos de ADN separados por electroforesis y transferidos a una membrana. ​

 
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