immunoassay and protein assayOnline version protein microarray by munirah mihat 1 How do heterogeneous assays differ from homogeneous assays? a Heterogeneous assays require a separation step. b Heterogeneous assays are easier to perform than homogeneous assays. c The concentration of patient analyte is directly proportional to bound label in homogeneous assays d Homogeneous assays are more sensitive than heterogeneous ones. 2 Which of the following best characterizes chemiluminescent assays? a Only the antigen can be labeled. b Tests can be read manually. c These are only homogeneous assays. d A chemical is oxidized to produce light. 3 Which of these concerns would apply to functional protein microarrays but not antibody microarrays? a Antibodies recognize relatively short peptide sequences from a protein, and presence of the same peptide sequence in several proteins is possible. b Three dimensional structure of a protein is essential for correct function, and the process of microarray preparation may interfere with this structure. c Certain functions, such as structural support of a cell, may not be amenable to analysis on a microarray d All of these apply to functional protein microarrays but not antibody microarrays 4 When using an enzyme immunoassay to study microtubules or other structures inside a cell, we first chemically fix the cell and then treat the cells with alcohol. What is the purpose of this alcohol treatment? a is bound by the antibody’s antigen-binding site. b is attached to the well of a microtiter plate. c is conjugated to the suspect antigen. d is bound to the constant region of the secondary antibody. 5 Which of the following is immobilized on the microtiter well in sandwich ELISA? a detection antibody b sample c capture antibody d secondary antibody conjugated to an enzyme
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