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(62)
PART 2

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About Quiz
blotting
molecular biology
 Recommended age: 19 years old
252 times made

Created by

munirah mihat
munirah mihat
Malaysia

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PART 2

by munirah mihat
1

After proteins are run on an SDS-PAGE gel, a transfer is executed. What is the purpose of the transfer in Western blot protocol?

2

Which of the following probes are most commonly used in Western blotting?

3

A cell line was engineered to contain and express a DNA sequence frequently associated with pancreatic cancer, with the hope that the cell line will undergo rapid cell division much like the cancerous cells. You want to test whether or not the cell line is efficiently transcribing this DNA by measuring the quantity of mRNA produced. Which of the following methods would be best suited for testing this?

4

In a Western Blot, the primary antibody binds to the ________ while the secondary antibody binds to the _______.

5

What is the purpose of "blocking" the nitrocellulose membrane during western blotting?

 
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