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Subcellular Fractionation Quiz

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Subcellular Fractionation Quiz

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About this activity

Cell component separation basics

Created by

Dr.Ruby Varghese
Dr.Ruby Varghese
India

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Subcellular Fractionation Quiz
 

Subcellular Fractionation QuizOnline version

Cell component separation basics

by Dr.Ruby Varghese
1

Which centrifugation method is primarily used for this separation?

2

Name one property exploited to separate organelles.

3

Which condition is typically used during subcellular fractionation?

4

Which media is commonly used in differential centrifugation?

5

Which of the following is NOT a property used for separation?

6

Which other media may be used besides sucrose in differential centrifugation?

7

What property is not typically used as a separation parameter?

8

Ficoll 400 is mentioned as a medium; what is its role?

9

Why is the process done at high viscosity media?

10

Which centrifugation method separates by size and yields crude fractions as the first step in fractionation?

11

Which interactions are disrupted to solubilize proteins?

12

What is exposed when solubilizing hydrophobic regions?

13

Which factor guides the solubilization method?

14

Name a common solubilization agent?

15

Which class of agents disrupts hydrogen bonds non-specifically?

16

Which approach is used to solubilize proteins with high hydrophobicity?

17

What is a typical role of detergents in solubilization?

18

Why might a salt aid solubilization?

19

Which method is unsuitable for all proteins?

20

What should be considered when selecting a solubilization method?

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Differential centrifugation separates components by size and density in steps.

Buoyant density differences allow sequential pelleting of components.

Low temperature preserves organelle integrity and enzyme activity.

Sucrose solutions provide controlled density for gradient/viscosity adjustments.

Color is not a physical property used for fractionation.

Mannitol is a common osmotic stabilizer in fractionation protocols.

Thermal conductivity is not a standard parameter for organelle separation.

Ficoll 400 increases medium viscosity to aid differential separation.

Higher viscosity helps achieve sharper separations during centrifugation.

Differential uses size-based sedimentation to fractionate cells/organelles; density-based methods separate by density rather than crude size fractions.

Solubilization targets non-covalent protein interactions that hold structure together.

Solubilizing often involves exposing hydrophobic cores to solvent to disrupt aggregation.

Choice depends on the protein’s size, charge, and hydrophobicity.

Detergents are widely used to disrupt hydrophobic interactions.

Chaotropes destabilize structured water and macromolecular interactions.

Organic solvents can better dissolve highly hydrophobic proteins.

Detergents form micelles around hydrophobic regions to keep proteins soluble.

Salts modulate electrostatic interactions, aiding solubility in many cases.

No universal method; choice depends on protein properties.

Matching method to protein properties improves solubility and yield.

 
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