Quiz: PG Induction MCQ Series


PG Induction MCQ SeriesOnline version Biochemicals by JR1 PG 1 For catalase test we use how much percentage of h2o2 can be used ? a 0.1 b 0.3 c 3 d 1 2 Catalase test can be performed from blood agar a true b false 3 Tube coagulase final reading done at ? a 1hr b 2hr c 4hr d 24hr 4 For VP test Reagents used is ? a MR b Barrit's c Kovacs d Albert 5 We performed Oxidase test from NLF colonies of Mackonkay Agar . We reported it as Oxidase positive. Is it true positive or false positive ? a True Positive b False Positive 6 Nichrome wire can be used for oxidase test – true or false a true b false 7 In which Biochemical test is stabbing done? a catalase b urease c citrate d TSI 8 Which broth is used for MR ? a Albert b GP Broth c Barrit's d Kovacs 9 TSI agar shows which of the following a Gas production b H2S production c Sugar fermentation d All of the above 10 Coagulase test uses what? a plasma b whole blood c serum d all of the above Feedback 1 3% h202 is the standard concentration for most aerobic bacteria. However, for Anaerobes, a higher concentration (15% h202) is sometimes used to detect the weaker catalase production in those organisms 2 Red blood cells contain their own catalase. If you accidentally pick up the agar along with the colony, the h202 will react with the blood in the medium, giving a False Positive. It is best performed on Nutrient Agar or Heart Infusion Agar. 3 While you check the tube every 4 hours (looking for "free coagulase"), some strains of S. aureus produce Staphylokinase (fibrinolysin), which can dissolve a clot that formed early. A final negative should only be reported after 24 hours of incubation at room temperature to ensure a late-forming clot wasn't missed or dissolved. 4 Barritt’s Reagent A is $\alpha$-naphthol and Reagent B is KOH (40%). Together they detect acetoin (acetylmethylcarbinol).Kovacs is for Indole; Albert is a stain for C. diphtheriae. 5 You should never perform an oxidase test from MacConkey agar. The indicators/dyes (Neutral Red and Crystal Violet) in the medium can interfere with the color reaction of the oxidase reagent (tetramethyl-p-phenylenediamine dihydrochloride), leading to false results. Use a non-selective medium like Sheep Blood Agar. 6 Nichrome or Iron-based loops can catalyze the oxidation of the reagent itself, leading to a False Positive. Always use a glass rod, plastic loop, or a wooden applicator stick. 7 TSI (Triple Sugar Iron) requires both an aerobic environment (the slant) and an anaerobic environment (the butt). You stab the butt and then streak the slant. Citrate and Urease are usually streaked only on the slant. 8 The full name is Glucose Phosphate Broth (also known as MR-VP Medium). It provides the glucose necessary for the "mixed acid fermentation" pathway that the MR test detects. 9 TSI is a multi-purpose medium. It detects: Sugar fermentation: Glucose (butt), Lactose/Sucrose (slant).Gas: Bubbles or cracking of the medium. H2S production: Blackening of the medium due to Ferrous Sulfate. 10 You need Fibrinogen for the test to work (Coagulase converts Fibrinogen to Fibrin). Serum does not contain clotting factors, and whole blood obscures the visual of the clot. Rabbit plasma with EDTA is the gold standard.

1

For catalase test we use how much percentage of h2o2 can be used ?

a

0.1

b

0.3

c

3

d

1

2

Catalase test can be performed from blood agar

a

true

b

false

3

Tube coagulase final reading done at ?

a

1hr

b

2hr

c

4hr

d

24hr

4

For VP test Reagents used is ?

a

MR

b

Barrit's

c

Kovacs

d

Albert

5

We performed Oxidase test from NLF colonies of Mackonkay Agar . We reported it as Oxidase positive. Is it true positive or false positive ?

a

True Positive

b

False Positive

6

Nichrome wire can be used for oxidase test – true or false

a

true

b

false

7

In which Biochemical test is stabbing done?

a

catalase

b

urease

c

citrate

d

TSI

8

Which broth is used for MR ?

a

Albert

b

GP Broth

c

Barrit's

d

Kovacs

9

TSI agar shows which of the following

a

Gas production

b

H2S production

c

Sugar fermentation

d

All of the above

10

Coagulase test uses what?

a

plasma

b

whole blood

c

serum

d

all of the above

Feedback

1

3% h202 is the standard concentration for most aerobic bacteria. However, for Anaerobes, a higher concentration (15% h202) is sometimes used to detect the weaker catalase production in those organisms

2

Red blood cells contain their own catalase. If you accidentally pick up the agar along with the colony, the h202 will react with the blood in the medium, giving a False Positive. It is best performed on Nutrient Agar or Heart Infusion Agar.

3

While you check the tube every 4 hours (looking for "free coagulase"), some strains of S. aureus produce Staphylokinase (fibrinolysin), which can dissolve a clot that formed early. A final negative should only be reported after 24 hours of incubation at room temperature to ensure a late-forming clot wasn't missed or dissolved.

4

Barritt’s Reagent A is $\alpha$-naphthol and Reagent B is KOH (40%). Together they detect acetoin (acetylmethylcarbinol).Kovacs is for Indole; Albert is a stain for C. diphtheriae.

5

You should never perform an oxidase test from MacConkey agar. The indicators/dyes (Neutral Red and Crystal Violet) in the medium can interfere with the color reaction of the oxidase reagent (tetramethyl-p-phenylenediamine dihydrochloride), leading to false results. Use a non-selective medium like Sheep Blood Agar.

6

Nichrome or Iron-based loops can catalyze the oxidation of the reagent itself, leading to a False Positive. Always use a glass rod, plastic loop, or a wooden applicator stick.

7

TSI (Triple Sugar Iron) requires both an aerobic environment (the slant) and an anaerobic environment (the butt). You stab the butt and then streak the slant. Citrate and Urease are usually streaked only on the slant.

8

The full name is Glucose Phosphate Broth (also known as MR-VP Medium). It provides the glucose necessary for the "mixed acid fermentation" pathway that the MR test detects.

9

TSI is a multi-purpose medium. It detects: Sugar fermentation: Glucose (butt), Lactose/Sucrose (slant).Gas: Bubbles or cracking of the medium. H2S production: Blackening of the medium due to Ferrous Sulfate.

10

You need Fibrinogen for the test to work (Coagulase converts Fibrinogen to Fibrin). Serum does not contain clotting factors, and whole blood obscures the visual of the clot. Rabbit plasma with EDTA is the gold standard.

You can integrate the game into an LMS compatible with LTI 1.1 or LTI 1.3 such as Canvas, Moodle, or Blackboard. This way, the scores will be automatically saved into the platform’s gradebook.

PG Induction MCQ Series

Quiz

About this activity

Created by

Download the paper version to play

Top Games

Quiz

Photosynthesis vs Cellular Respiration - Practice Quiz

Quiz

Spanish Alphabet Listening

Quiz

UPS 5 Seeing Habits Quiz

Quiz

Tissues - Practice Quiz

Quiz

Practice Quiz - Punnett Square, Variation, and Mutations

PG Induction MCQ SeriesOnline version

by JR1 PG

For catalase test we use how much percentage of h2o2 can be used ?

Catalase test can be performed from blood agar

Tube coagulase final reading done at ?

For VP test Reagents used is ?

We performed Oxidase test from NLF colonies of Mackonkay Agar . We reported it as Oxidase positive. Is it true positive or false positive ?

Nichrome wire can be used for oxidase test – true or false

In which Biochemical test is stabbing done?

Which broth is used for MR ?

TSI agar shows which of the following

Coagulase test uses what?

Feedback

		%
	Anonymous	%
		%
		%