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PG Induction MCQ Series

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PG Induction MCQ Series

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Quality Control

Created by

JR1 PG
JR1 PG
India

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PG Induction MCQ Series
 

PG Induction MCQ SeriesOnline version

Quality Control

by JR1 PG
1

Gram stain shows all bacteria pink. What to do?

2

QC strain E.coli ATCC 25922 shows no zone for Ampicillin. Action?

3

Blood agar batch shows contamination. Next steps?

4

Antibiotic discs expired 1 month ago. Use or not?

5

AST report shows S.aureus sensitive to Penicillin (control S.aureus ATCC 25923 resistant). Verify?

Feedback

Clarification: If known Gram-positive organisms (like Staphylococci) appear pink, the most common culprit is over-decolorization. However, it could also be that the Crystal Violet has deteriorated or the Safranin is too concentrated. Troubleshooting Step: You must check the decolorizer (ensure it isn't pure acetone if you usually use an alcohol-acetone mix) and repeat the stain with fresh reagents and a known QC slide (e.g., S. aureus and E. coli on the same slide).

Clarification: E. coli ATCC 25922 is the standard sensitive QC strain. It must show a zone. No zone indicates a major failure. Troubleshooting Step: * Disc Potency: The Ampicillin disc may have lost its potency due to improper storage (moisture/heat). Media pH: If the Mueller-Hinton Agar (MHA) pH is too acidic, it can interfere with the diffusion of certain antibiotics. Reporting: You cannot report any patient results from this batch until the QC issue is resolved.

Clarification: Contamination in a new batch of media usually points to an Autoclave failure (sterilization issue) or poor aseptic technique when adding the sheep blood (which is added after the agar base cools to 45-50 C . Action: You cannot "subculture" or "use" contaminated media as it will lead to false growth and misdiagnosis. The entire batch is compromised.

Clarification: In an ISO-accredited or standard clinical lab (like yours at GMC), using expired reagents is a major non-conformity. The "Why": Antibiotic discs (especially beta-lactams like Penicillin or Ampicillin) are highly sensitive to humidity. Even if they "seem" to work, their concentration is no longer guaranteed, which can lead to a False Resistant report for a patient.

Clarification: This is a classic "Red Flag." True Penicillin-sensitive S. aureus is now rare (<5-10% in most areas). If your patient isolate is sensitive but your QC strain (which is supposed to be sensitive or have a known range) shows resistance, the test is invalid.Verification: You must check if the discs were expired or if the inoculum density (McFarland standard) was too light.Key Point: For S. aureus, you should also check the Zone Edge. A "fuzzy" or "feathered" edge suggests it is truly sensitive, while a "sharp" or "cliff-like" edge suggests beta-lactamase production (even if the zone size looks large).

 
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