Quiz: Biologia Estructural 2


Biologia Estructural 2Online version Pres 11 - 12 by __________@# 1 1. What is the primary function of the eEF1A protein in the eukaryotic elongation cycle? a A) Catalyzing peptide bond formation at the P-site. b B) Recycling GDP to GTP through its own intrinsic activity. c C) Delivering aminoacyl-tRNA (aa-tRNA) to the ribosomal A-site. d D) Acting as a termination factor to release the polypeptide chain. e E) Translocating the mRNA exactly three nucleotides forward. 2 2. During nucleotide exchange, what specific interaction occurs in Switch 2 following the insertion of eEF1Bα? a A) Switch 2 is stabilized by a water molecule binding to Mg2+. b B) Asp-91a is displaced and forms a salt bridge with Lys-20a of the P-loop. c C) Helix B moves to seal the nucleotide-binding pocket. d D) Switch 2 interacts directly with the adenine base of ATP. e E) Lys-205b forms a covalent bond with the γ-phosphate. 3 3. Which domain(s) interact with the Sarcin/Ricin Loop “SRP” of the ribosome? a A) Domains 1 and 3. b B) Domains 2 and 3. c C) Domain 2. d D) Domains 1, 2, and 3. e E) Domains 1 and 2. 4 4. The "NKMD" motif in eEF1A is a specific set of amino acids that recognizes which part of the nucleotide? a A) The adenine base. b B) The guanine base. c C) The phosphate tail only. d D) The water molecules. e E) The ribosome backbone. 5 5. Which structural element of eEF1A helps coordinate the decoding process? a A) The P-loop. b B) The α2 helix, which interacts with intersubunit bridge B8. c C) A and B are correct. d D) The β-strands of the Domain 2 barrel. e E) All above are correct. 6 6. What phenomenon explains the competition between eEF1Ba and tRNA? a A) pH changes b B) Interaction with ribosomes c C) Overlap of their binding sites on eEF1A d D) Degradation of tRNA e E) None of the above is correct. 7 7. What is the role of the subunit "rolling" in the eukaryotic ribosome? a A) It is a rotary movement that permanently expels eEF1A. b B) It is the process by which the ribosome detaches from the ER membrane. c C) A and B are true. d D) It serves to move the A-site tRNA to the E-site without passing through the P-site. e E) None of the above is correct. 8 8. The competition for Domain 2 is biologically significant because it ensures that: a A) eEF1Bα only leaves eEF1A when a new aa-tRNA is ready to be loaded. b B) GTP is never used by the elongation factor. c C) The ribosome can only bind one tRNA at a time. d D) The G-domain is always in an inactive GDP state. e E) Amino acids are only attached to the 5’ end of the tRNA. 9 9. According to structural analysis of the eEF1A:eEF1Bα complex, what is the role of Gln-204b? a A) It forms a salt bridge with the γ-phosphate of GTP. b B) It acts as a "wedge" by inserting between two antiparallel β-strands connecting Switch 1 and Switch 2. c C) It coordinates the Mg2+ion in the inactive state. d D) It stabilizes the binding of the tRNA CCA-end. e E) C and D are correct. 10 10. To which site of the ribosome is the aminoacyl-tRNA (aa-tRNA) delivered when it is carried by EEF1A? a A) The P site (Peptidyl site). b B) The E site (Exit site). c C) The A site (Aminoacyl site). d D) The Z site (Noncanonical site). e E) The Sarcin-Ricin Loop (SRL). 11 Which fold has the RecA domain from Brr2? a rossmann fold b greek-key c beta propeller d all alpha helix bundle e ____ 12 The Q motif is capable of binding… a The N6 of the ATP b The N7 of the ATP c the two previous answers are correct (A i B) d The gamma phosphate e The beta phosphate 13 Which is the mutation responsible for the incapacity of the C-cassette to hydrolyze ATP? a G1353N b G1692N c G452T d N1284G e N1983G 14 Identify the Brr2 mutation responsible for Retinitis Pigmentosa 33 that exhibits a significant energetic shift compared to the wild-type protein. a A561L b R681A c R681C d R732C e R732V 15 Which coordination number does magnesium have in Brr2 of the spliceosoma? a 14 b 6 c 9 d 2 e 1 16 Which family does Brr2 belong to? a DEAD box b SF2 c Ski-2 like d Hemagglutinin e Kinases 17 How does the RH domain of Prp8 inhibit Brr2? a by physical inhibition only b when Jab1/MPN domain is not inhibiting c the two previous are correct (A i B) d by physical inhibition and allosteric inhibition e all the above are correct 18 Which of these subunits is not a component of the spliceosome? a U1 b U2 c U3 d U4 e U5 19 The C cassette has the function of: a catalytic domain b Regulatory domain c Forms the tunnel where the RNA is going to enter d Structural support of the N cassette e Inhibitory by blocking the entrance of RNA in the tunnel. 20 Which motifs are in charge of binding and hydrolize the ATP: a I,II, III b I, III, IV c I, III, V d I, II, VI e I, II, IV

1

1. What is the primary function of the eEF1A protein in the eukaryotic elongation cycle?

a

A) Catalyzing peptide bond formation at the P-site.

b

B) Recycling GDP to GTP through its own intrinsic activity.

c

C) Delivering aminoacyl-tRNA (aa-tRNA) to the ribosomal A-site.

d

D) Acting as a termination factor to release the polypeptide chain.

e

E) Translocating the mRNA exactly three nucleotides forward.

2

2. During nucleotide exchange, what specific interaction occurs in Switch 2 following the insertion of eEF1Bα?

a

A) Switch 2 is stabilized by a water molecule binding to Mg2+.

b

B) Asp-91a is displaced and forms a salt bridge with Lys-20a of the P-loop.

c

C) Helix B moves to seal the nucleotide-binding pocket.

d

D) Switch 2 interacts directly with the adenine base of ATP.

e

E) Lys-205b forms a covalent bond with the γ-phosphate.

3

3. Which domain(s) interact with the Sarcin/Ricin Loop “SRP” of the ribosome?

a

A) Domains 1 and 3.

b

B) Domains 2 and 3.

c

C) Domain 2.

d

D) Domains 1, 2, and 3.

e

E) Domains 1 and 2.

4

4. The "NKMD" motif in eEF1A is a specific set of amino acids that recognizes which part of the nucleotide?

a

A) The adenine base.

b

B) The guanine base.

c

C) The phosphate tail only.

d

D) The water molecules.

e

E) The ribosome backbone.

5

5. Which structural element of eEF1A helps coordinate the decoding process?

a

A) The P-loop.

b

B) The α2 helix, which interacts with intersubunit bridge B8.

c

C) A and B are correct.

d

D) The β-strands of the Domain 2 barrel.

e

E) All above are correct.

6

6. What phenomenon explains the competition between eEF1Ba and tRNA?

a

A) pH changes

b

B) Interaction with ribosomes

c

C) Overlap of their binding sites on eEF1A

d

D) Degradation of tRNA

e

E) None of the above is correct.

7

7. What is the role of the subunit "rolling" in the eukaryotic ribosome?

a

A) It is a rotary movement that permanently expels eEF1A.

b

B) It is the process by which the ribosome detaches from the ER membrane.

c

C) A and B are true.

d

D) It serves to move the A-site tRNA to the E-site without passing through the P-site.

e

E) None of the above is correct.

8

8. The competition for Domain 2 is biologically significant because it ensures that:

a

A) eEF1Bα only leaves eEF1A when a new aa-tRNA is ready to be loaded.

b

B) GTP is never used by the elongation factor.

c

C) The ribosome can only bind one tRNA at a time.

d

D) The G-domain is always in an inactive GDP state.

e

E) Amino acids are only attached to the 5’ end of the tRNA.

9

9. According to structural analysis of the eEF1A:eEF1Bα complex, what is the role of Gln-204b?

a

A) It forms a salt bridge with the γ-phosphate of GTP.

b

B) It acts as a "wedge" by inserting between two antiparallel β-strands connecting Switch 1 and Switch 2.

c

C) It coordinates the Mg2+ion in the inactive state.

d

D) It stabilizes the binding of the tRNA CCA-end.

e

E) C and D are correct.

10

10. To which site of the ribosome is the aminoacyl-tRNA (aa-tRNA) delivered when it is carried by EEF1A?

a

A) The P site (Peptidyl site).

b

B) The E site (Exit site).

c

C) The A site (Aminoacyl site).

d

D) The Z site (Noncanonical site).

e

E) The Sarcin-Ricin Loop (SRL).

11

Which fold has the RecA domain from Brr2?

a

rossmann fold

b

greek-key

c

beta propeller

d

all alpha helix bundle

e

____

12

The Q motif is capable of binding…

a

The N6 of the ATP

b

The N7 of the ATP

c

the two previous answers are correct (A i B)

d

The gamma phosphate

e

The beta phosphate

13

Which is the mutation responsible for the incapacity of the C-cassette to hydrolyze ATP?

a

G1353N

b

G1692N

c

G452T

d

N1284G

e

N1983G

14

Identify the Brr2 mutation responsible for Retinitis Pigmentosa 33 that exhibits a significant energetic shift compared to the wild-type protein.

a

A561L

b

R681A

c

R681C

d

R732C

e

R732V

15

Which coordination number does magnesium have in Brr2 of the spliceosoma?

a

14

b

6

c

9

d

2

e

1

16

Which family does Brr2 belong to?

a

DEAD box

b

SF2

c

Ski-2 like

d

Hemagglutinin

e

Kinases

17

How does the RH domain of Prp8 inhibit Brr2?

a

by physical inhibition only

b

when Jab1/MPN domain is not inhibiting

c

the two previous are correct (A i B)

d

by physical inhibition and allosteric inhibition

e

all the above are correct

18

Which of these subunits is not a component of the spliceosome?

a

U1

b

U2

c

U3

d

U4

e

U5

19

The C cassette has the function of:

a

catalytic domain

b

Regulatory domain

c

Forms the tunnel where the RNA is going to enter

d

Structural support of the N cassette

e

Inhibitory by blocking the entrance of RNA in the tunnel.

20

Which motifs are in charge of binding and hydrolize the ATP:

a

I,II, III

b

I, III, IV

c

I, III, V

d

I, II, VI

e

I, II, IV

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Biologia Estructural 2

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Biologia Estructural 2Online version

by __________@#

1. What is the primary function of the eEF1A protein in the eukaryotic elongation cycle?

2. During nucleotide exchange, what specific interaction occurs in Switch 2 following the insertion of eEF1Bα?

3. Which domain(s) interact with the Sarcin/Ricin Loop “SRP” of the ribosome?

4. The "NKMD" motif in eEF1A is a specific set of amino acids that recognizes which part of the nucleotide?

5. Which structural element of eEF1A helps coordinate the decoding process?

6. What phenomenon explains the competition between eEF1Ba and tRNA?

7. What is the role of the subunit "rolling" in the eukaryotic ribosome?

8. The competition for Domain 2 is biologically significant because it ensures that:

9. According to structural analysis of the eEF1A:eEF1Bα complex, what is the role of Gln-204b?

10. To which site of the ribosome is the aminoacyl-tRNA (aa-tRNA) delivered when it is carried by EEF1A?

Which fold has the RecA domain from Brr2?

The Q motif is capable of binding…

Which is the mutation responsible for the incapacity of the C-cassette to hydrolyze ATP?

Identify the Brr2 mutation responsible for Retinitis Pigmentosa 33 that exhibits a significant energetic shift compared to the wild-type protein.

Which coordination number does magnesium have in Brr2 of the spliceosoma?

Which family does Brr2 belong to?

How does the RH domain of Prp8 inhibit Brr2?

Which of these subunits is not a component of the spliceosome?

The C cassette has the function of:

Which motifs are in charge of binding and hydrolize the ATP:

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