Clinical Laboratory Procedures: Gram Stain & ELISA TestOnline version
Student-made practice quiz for VA students of PIMA Medical Institute. Includes study material for Sequence I: Clinical Laboratory Procedures- Module 3, Hematology, Gram Stainging, and ELISA Testing.
1
What color is gram positive bacteria after staining?
a
Blue/Purple
b
Pink/Red
c
Yellow/Green
d
Green/Blue
2
What color is gram negative bacteria after staining?
a
Blue/Purple
b
Pink/Red
c
Yellow/Green
d
Green/Blue
3
What kind of bacteria is described as sphere-shaped?
4
What kind of bacteria is described as rod-shaped?
5
What kind of bacteria is described as spiral-shaped?
6
What are the four main types of agars for growing bacteria?
a
Blood, MacConkey, Nutrient, Mueller
b
Blood, Platelet, Nutrient, Protein
c
MacConkey, Protein, Mueller, Platelet
d
MacConkey, Mueller, Roeder, Adson
7
Why is gram staining performed?
a
To differentiate and view the different types of bacteria present in a sample.
b
To kill bacteria in a sample for better research and storage without risk of exposing the viewers.
c
To enhance cell structions so you can evaluate morphology estimate platelets, and identify abnormalisities that may not show up in automated analyzers.
d
To evaluate the cellular components of blood.
8
How does the information we obtain from gram staining help in the diagnosis and treatment of a patient?
a
it provides knowledge on the type of bacteria in a sample and help the doctor make an effective treatment plan
b
Better understanding of the cellular morphology will assist in diagnosing the patient.
c
To view the presence of microscopic parasites, antibodies, and antigens in the bloodstream to better understand the symptoms the patient is experiencing
d
Gram staining is not used for diagnosis, but is instead used to research already diagnosed conditions and only used to adapt methods for better treatment
9
What type of sample is needed for a heartworm ELISA test?
a
Plasma
b
Serum
c
Whole Blood
d
Whole Blood, Serum, and/or Plasma
e
Feces
f
Sliva/Spit
10
What type of sample is needed for a FIV/FeLV ELISA test?
a
Plasma
b
Serum
c
Whole Blood
d
Whole Blood, Serum, and/or Plasma
e
Feces
f
Sliva/Spit
11
What type of sample is needed for a Parvovirus ELISA test?
a
Plasma
b
Serum
c
Whole Blood
d
Whole Blood, Serum, and/or Plasma
e
Feces
f
Sliva/Spit
12
What type of sample is needed for a Giardia ELISA test?
a
Plasma
b
Serum
c
Whole Blood
d
Whole Blood, Serum, and/or Plasma
e
Feces
f
Sliva/Spit
13
What temperature does bacteria incubate at?
a
88.5 Degrees (Fahrenheit)
b
98.6 Degrees (Fahrenheit)
c
100.3 Degrees (Fahrenheit)
d
Room Temperature
14
What temperature does a fungal culture incubate at?
a
88.5 Degrees (Fahrenheit)
b
98.6 Degrees (Fahrenheit)
c
100.3 Degrees (Fahrenheit)
d
Room Temperature
15
What PPE is REQUIRED when using a tease mount for a fungal culture?
a
Gloves, face mask, eye protection
b
Gloves, face mask, lab coat
c
Gloves, eye protection, lab coat
d
Face mask, eye protection, lab coat
16
What is the first step to a gram stain?
a
Heat fix the slide, with the sample already present, by passing it over the flame
b
Use crystal violet stain
c
Use Iodine to fix the sample
d
Rinse the slide
17
What order are the stains used during a gram stain?
a
Crystal Violet; Iodine; Decolorizor; Counter Stain
b
Iodine; Crystal Violet; Counter Stain; Decolorizor
c
Crystal Violet; Decolorizor; Counter Stain; Iodine
d
Decolorizor; Crystal Violet; Counter Stain; Iodine
18
What step is missing:
Heat Fix; Crystal Violet; Rinse; ___________; Rinse; Decolorizor; Counter Stain; Rinse; Dry
19
What step is missing:
Heat Fix; _______; Rinse; Iodine; Rinse; Decolorizor; Counter Stain; Rinse; Dry
20
What step is missing:
Heat Fix; Crystal Violet; Rinse; Iodine; Rinse; Decolorizor; __________; Rinse; Dry
21
What step is missing:
Heat Fix; Crystal Violet; Rinse; Iodine; Rinse; _________; Counter Stain; Rinse; Dry
Photosynthesis vs Cellular Respiration - Practice Quiz