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Fundamentos de UV-Vis

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Fundamentos de UV-Vis

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Quiz sobre fundamentos básicos de UV-Vis

Created by

Maria Echaiz Veliz
Maria Echaiz Veliz
Peru

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Fundamentos de UV-Vis
 

Fundamentos de UV-VisOnline version

Quiz sobre fundamentos básicos de UV-Vis

by Maria Echaiz Veliz
1

¿Qué mide la espectrofotometría UV-Visible?

2

¿Qué ley relaciona absorbancia, concentración y ruta óptica?

3

Qué es un blanco (blank) en espectrofotometría?

4

¿Qué representa la transmittancia (T)?

5

Factores que pueden afectar medidas UV-Vis?

Explicación

La técnica cuantifica cuánta luz es absorbida por la muestra a diferentes longitudes de onda.

La Ley de Beer-Lambert conecta absorbancia, concentración y longitud de camino, con molar absorptividad.

El blanco corrige la influencia del solvente y de la cubeta.

T es la fracción de luz que pasa, A=(-log T).

Solventes, color del fondo y partículas pueden sesgar resultados si no se corrigen.

 
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