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Gene Expression Challenge

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Gene Expression Challenge

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Test your knowledge of transcription, translation, and regulation

Created by

Isabelle Shiraishi
Isabelle Shiraishi
United States

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Gene Expression Challenge
 

Gene Expression ChallengeOnline version

Test your knowledge of transcription, translation, and regulation

by Isabelle Shiraishi
1

Which promoter elements are typical for prokaryotes?

2

Which core promoter element is characteristic of eukaryotic transcription?

3

What happens when LacI repressor binds the lac operator (O)?

4

In the presence of lactose, what acts as the inducer to relieve LacI repression?

5

What regulatory mechanism uses a leader peptide to terminate transcription in some cells?

6

Which molecular chaperones are commonly involved in protein folding?

7

Name a common post-translational modification listed in the slides.

8

What is the insulin processing pathway as described?

9

What is meant by “alternative” promoters in gene regulation?

10

Which regulator provides catabolite repression of the lac operon?

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Prokaryotic promoters commonly have a -10 Pribnow box and -35 region.

Eukaryotic core promoters often include a TATA box recognized by TBP.

LacI binding to O represses transcription of lac operon genes.

Allolactose binds LacI causing dissociation from the operator; IPTG is a lab inducer.

Attenuation in the trp operon uses a leader peptide to terminate transcription under certain tryptophan levels.

Chaperones like Hsp70/Hsp40 and GroEL/GroES assist folding; Hsp90 also participates.

Post-translational modifications include ubiquitination among others.

Preproinsulin is processed to proinsulin, then to active insulin with disulfide bonds.

Alternative promoters provide tissue- or time-specific expression.

CRP-cAMP binding stimulates transcription when glucose is low, relieving repression.

 
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