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Basics of Molecular Biology & Sequencing

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Basics of Molecular Biology & Sequencing

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Molecular biology quiz

Created by

nora shebl
nora shebl
Egypt

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Basics of Molecular Biology & Sequencing
 

Basics of Molecular Biology & SequencingOnline version

Molecular biology quiz

by nora shebl
1

Which three biomolecule types are central to cellular function?

2

What is the flow of genetic information called in biology?

3

Which enzyme copies DNA during replication?

4

What is a codon?

5

How do prokaryotes and eukaryotes differ in DNA organization?

6

Which sequencing method uses ddNTPs to terminate DNA synthesis?

7

What read length is typical for Illumina sequencing platforms?

8

Why are long repeats a problem for genome assembly?

9

What is BAC-by-BAC sequencing?

10

What is a contig in sequence assembly?

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DNA stores information, RNA transfers/ templates, proteins do the work.

Transcription transfers info from DNA to RNA, translation makes protein.

DNA polymerase synthesizes new DNA strands using a template.

Triplets encode amino acids; there are 64 possible codons.

Prokaryotic DNA is typically a circular chromosome; eukaryotic DNA is linear and in nucleus.

Sanger (dideoxy) sequencing uses chain-terminating ddNTPs.

Illumina reads are short, usually tens of bases long per read.

When repeats longer than reads exist, correct placement is hard.

Each BAC is sequenced; assembly then merges BAC contigs.

Contigs result from merging reads that overlap to form longer sequences.

 
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