Quiz: Gen Micro


Gen MicroOnline version Part 2 by Consistency 1 Which of the following indicates a positive presumptive presence of Salmonella using Xylose Lysine Deoxycholate agar after incubation at 37°C for 24hrs? a Pink colonies with or without black centers b Blue-green to blue colonies with or without centers c Brown, gray, or black colonies d Appear as opaque/yellow, pinkor red colonies with black centers 2 What is the purpose of using a selective agar in microbial plating? a To inhibit the growth of unwanted microorganisms. b To encourage the growth of specific types of microorganisms. c To allow the growth of a wide range of microorganisms.. d To neutralize the pH of the sample 3 What is the purpose of staining a biological sample before observing it under a microscope? a To make the sample more visible under the microscope. b To change the chemical composition of the sample. c To kill the cells in the sample. d To make the sample more durable for long-term storage. 4 Ernst August Friedrich Ruska was a German physicist who won the Nobel Prize in Physics in 1986. He is known to have invented the first_________. a Electon microscope b Simple microscope c Compound microscope d Flourescence microscope 5 What is the acceptable range of viable counts per plate, as specified by the Bacteriological Analytical Manual (BAM), for calculating colony-forming units for yeast and molds? a 10-150 colonies b 10-250 colonies c 15-150 colonies d 25-250 colonies 6 Penicillin blocks cell wall synthesis. During which phase of the growth cycle would a population of Streptococcus be most sensitive to penicillin? a Log Phase b Lag Phase c Stationary Phase d Death Phase 7 How does non-ionizing radiation exert its antimicrobial effect? a By Inducing DNA damage. b By disrupting the cell membrance c By inhibiting protein synthesis d By altering membrane potential 8 Salmonella has a generation time of 30minutes. If four cells are present on a turkey that is left on the counter, how many cells will be present after 3hours? a 256 b 12 c 24 d 54 9 Which colony appearance is most typical of Salmonella on Brilliant Green Agar? a Red to pink colonies surrounded by a red medium b Yellow-green colonies with yellow medium c Black colonies with metallic sheen d Blue-green colonies with fluorescence 10 A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. How many generations have passed? Calculate for the growth rate. a 17generations;3.23generations/hr b 17generations;0.05generations/min c 23generations;3.23generations/hr d 23generations;0.05generations/min 11 A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. What is the doubling time? a 0.35hours b 1.30hours c 0.95hours d 0.53hours 12 What is the color of the cells containing endospores after staining? a Green b Pink c Blue d Violet 13 The following results to a pink/red stain, except for: a Non-acid fast bacteria b Gram negative bacteria c Acid fast bacteria d Vegetative cells 14 A food microbiologist observes yellow colonies surrounded by yellow zones on Mannitol Salt Agar (MSA). Which interpretation is most appropriate? a The organism ferments mannitol, suggesting possible S.aureus b The organism produces hydrogen sulfide c The organism is confirmed as S.aureus d The organism is a Gram-negative coliform 15 Based on the Bacteriological Analytical Manual (BAM), what is the set valid count range per plate for computing the Colony Forming Units for Aerobic plate count? a 15-300 colonies b 15-150 colonies c 10-150 colonies d 20-250 colonies Feedback 5 BAM Chapter 18: Yeast, Molds, and Mycotoxins (10-150 colonies) 6 During log phase, bacteria actively divide and synthesize new cell walls, making them high sensitive to penicillin. 7 Non-ionizing radiation, such as ultraviolet (UV) light, exerts its anitmicrobial effect by damaging microbial DNA, specifically through formation of pyramidine dimers (thymine-thymine bonds). 8 G = 30 mins N1 = 4 cells t= 3 hours there is 6 30 minutes in 3 hours Sol. N2=4x2x2x2x2x2x2= 256cells

1

Which of the following indicates a positive presumptive presence of Salmonella using Xylose Lysine Deoxycholate agar after incubation at 37°C for 24hrs?

a

Pink colonies with or without black centers

b

Blue-green to blue colonies with or without centers

c

Brown, gray, or black colonies

d

Appear as opaque/yellow, pinkor red colonies with black centers

2

What is the purpose of using a selective agar in microbial plating?

a

To inhibit the growth of unwanted microorganisms.

b

To encourage the growth of specific types of microorganisms.

c

To allow the growth of a wide range of microorganisms..

d

To neutralize the pH of the sample

3

What is the purpose of staining a biological sample before observing it under a microscope?

a

To make the sample more visible under the microscope.

b

To change the chemical composition of the sample.

c

To kill the cells in the sample.

d

To make the sample more durable for long-term storage.

4

Ernst August Friedrich Ruska was a German physicist who won the Nobel Prize in Physics in 1986. He is known to have invented the first_________.

a

Electon microscope

b

Simple microscope

c

Compound microscope

d

Flourescence microscope

5

What is the acceptable range of viable counts per plate, as specified by the Bacteriological Analytical Manual (BAM), for calculating colony-forming units for yeast and molds?

a

10-150 colonies

b

10-250 colonies

c

15-150 colonies

d

25-250 colonies

6

Penicillin blocks cell wall synthesis. During which phase of the growth cycle would a population of Streptococcus be most sensitive to penicillin?

a

Log Phase

b

Lag Phase

c

Stationary Phase

d

Death Phase

7

How does non-ionizing radiation exert its antimicrobial effect?

a

By Inducing DNA damage.

b

By disrupting the cell membrance

c

By inhibiting protein synthesis

d

By altering membrane potential

8

Salmonella has a generation time of 30minutes. If four cells are present on a turkey that is left on the counter, how many cells will be present after 3hours?

a

256

b

12

c

24

d

54

9

Which colony appearance is most typical of Salmonella on Brilliant Green Agar?

a

Red to pink colonies surrounded by a red medium

b

Yellow-green colonies with yellow medium

c

Black colonies with metallic sheen

d

Blue-green colonies with fluorescence

10

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. How many generations have passed? Calculate for the growth rate.

a

17generations;3.23generations/hr

b

17generations;0.05generations/min

c

23generations;3.23generations/hr

d

23generations;0.05generations/min

11

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. What is the doubling time?

a

0.35hours

b

1.30hours

c

0.95hours

d

0.53hours

12

What is the color of the cells containing endospores after staining?

a

Green

b

Pink

c

Blue

d

Violet

13

The following results to a pink/red stain, except for:

a

Non-acid fast bacteria

b

Gram negative bacteria

c

Acid fast bacteria

d

Vegetative cells

14

A food microbiologist observes yellow colonies surrounded by yellow zones on Mannitol Salt Agar (MSA). Which interpretation is most appropriate?

a

The organism ferments mannitol, suggesting possible S.aureus

b

The organism produces hydrogen sulfide

c

The organism is confirmed as S.aureus

d

The organism is a Gram-negative coliform

15

Based on the Bacteriological Analytical Manual (BAM), what is the set valid count range per plate for computing the Colony Forming Units for Aerobic plate count?

a

15-300 colonies

b

15-150 colonies

c

10-150 colonies

d

20-250 colonies

Feedback

5

BAM Chapter 18: Yeast, Molds, and Mycotoxins (10-150 colonies)

6

During log phase, bacteria actively divide and synthesize new cell walls, making them high sensitive to penicillin.

7

Non-ionizing radiation, such as ultraviolet (UV) light, exerts its anitmicrobial effect by damaging microbial DNA, specifically through formation of pyramidine dimers (thymine-thymine bonds).

8

G = 30 mins N1 = 4 cells t= 3 hours there is 6 30 minutes in 3 hours Sol. N2=4x2x2x2x2x2x2= 256cells

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Gen Micro

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Gen MicroOnline version

by Consistency

Which of the following indicates a positive presumptive presence of Salmonella using Xylose Lysine Deoxycholate agar after incubation at 37°C for 24hrs?

What is the purpose of using a selective agar in microbial plating?

What is the purpose of staining a biological sample before observing it under a microscope?

Ernst August Friedrich Ruska was a German physicist who won the Nobel Prize in Physics in 1986. He is known to have invented the first_________.

What is the acceptable range of viable counts per plate, as specified by the Bacteriological Analytical Manual (BAM), for calculating colony-forming units for yeast and molds?

Penicillin blocks cell wall synthesis. During which phase of the growth cycle would a population of Streptococcus be most sensitive to penicillin?

How does non-ionizing radiation exert its antimicrobial effect?

Salmonella has a generation time of 30minutes. If four cells are present on a turkey that is left on the counter, how many cells will be present after 3hours?

Which colony appearance is most typical of Salmonella on Brilliant Green Agar?

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. How many generations have passed? Calculate for the growth rate.

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. What is the doubling time?

What is the color of the cells containing endospores after staining?

The following results to a pink/red stain, except for:

A food microbiologist observes yellow colonies surrounded by yellow zones on Mannitol Salt Agar (MSA). Which interpretation is most appropriate?

Based on the Bacteriological Analytical Manual (BAM), what is the set valid count range per plate for computing the Colony Forming Units for Aerobic plate count?

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