Quiz: Food Micro


Food MicroOnline version Part 2 by Consistency 1 What is the typical culture medium used in the MPN method for detecting coliforms in food and water? a Eosin Methylene Blue (EMB) Agar b Tryptic Soy Broth c Sorbitol MacConkey Agar d Lauryl Tryptose Broth (LTB) 2 In the process of microbial characterization, the laboratory analyst observed a distinct purple coloration in the results of the conducted gram staining. What classification of bacteria can be inferred to be present in the analyzed sample a Gram-positive b Gram-negative c Both A and B d Cannot be determined 3 The following results of biochemical test characterize Staphylococcus aureus, except: a Catalase negative b Coagulase positive c Thermonuclease positive d Glucose fermentation positive 4 Which culture medium is usually used for the isolation, cultivation and enumeration of lactic acid bacteria? a MRS (De Man, Rogosa and Sharpe) Agar b Potato Dextrose Agar c Dichloran rose bengal chloramphenicol (DRBC) agar d Eosin Methylene Blue (EMB) Agar 5 Which of the following microorganisms is considered very potent, doubling as fast as 10 minutes in optimum temperature? a Clostridium pefrigens b Staphylococcus aureus c Escherichia coli d Listeria monocytogenes 6 What are the IMViC results for E. coli? a +-+- b ++-- c +--- d --++ 7 The following type of media can be used in the identification of Salmonella spp. except a Blood Agar b Xylose lysine deoxycholate agar c Hektoen enteric agar d Bismuth sulfite agar 8 The following are examples of molecular methods for the detection and enumeration of foodborne microorganisms, except: a ELISA b PCR c DNA Sequencing d Rea-time PCR 9 Which of the following are not true about spoilage microorganisms? I. All spoilage microorganisms are also pathogenic microorganisms II. Spoilage microorganisms can contaminate the food only after processing III. Spoilage microorganisms produce substances that alter the sensory quality of foods IV. Spoilage microorganisms cannot mediate deterioration when the cells are killed byfood processing methods a I, II, IV only b I, II only c I, II, III only d II only 10 The following observations indicates a positive presence of E.coli, except: a produce green colonies, with or without metallic sheen b give IMViC patterns of ++--(biotype1) or -+-- (biotype2) c appear as Gram-negative nonsporeforming rods d ferment lactose with gas production 11 Microbial examination for drinking water shall provide the numbers/presence of the following microorganisms, except: a None of the above b Total coliform c Heterotrophic bacteria d E.coli/Thermotolerant coliform 12 In BAM, what is the most probable number (MPN) technique commonly used for? a Estimating low concentrations of coliforms and Eschirichia coli b Enumeration of viruses in food samples c Confirming toxin production in foodborne pathogens d Measuring pH in fermented foods 13 How many plate(s) should be used in reporting aerobic plate counts? a 2 b 1 c 4 d 3 14 How many plates of Baird-Parker Agar are use for the isolation and enumeration of Staphylococcus aureus a 3 b 2 c 1 d 4 15 In biochemical testing, Salmonella typically gives positive results in all of the following except a Phenol red lactose test b Polyvalent flagellar test c Polyvalent somatic test d Methyl red test 16 Escherichia coli is commonly found in the intestinal tract and can grow whether oxygen is present or absent, shifting its metabolism depending on environmental conditions. In terms of its oxygen requirement, what type of bacterium is E. coli? a Facultative anaerobe b Obligate anaerobe c Obligate aerobe d Microaerophile 17 This technique for plating is frequently employed to enumerated microorganisms in a heterogenous sample, which is introduced into molten agar medium before it solidifies a Pour plate technique b Spread plate technique c Streak plate technique d Enumeration method 18 What is the required number of sample units selected from lot of pasteurized milk that must be examined for Listeria monocytogenes? a 5 b 6 c 10 d 30 19 Typical characteristics of S. aureus and S. epidermidis are the following except for: a Bothe are positive on coagulase production b Both are positive on catalase activity c Bothe are positive on lysostaphin sensitivity d Both are positive on anaerobic utilization of glucose 20 Salmonella shows positive result in the following biochemical and seriological reactions except on: a Growth in KCN broth b Polyvalent flagellar test c Methyl red test d Purple color in lysine decarboxylase broth Feedback 8 Molecular methods PCR DNA Sequencing Real-time PCR 13 In APC it only use duplicate

1

What is the typical culture medium used in the MPN method for detecting coliforms in food and water?

a

Eosin Methylene Blue (EMB) Agar

b

Tryptic Soy Broth

c

Sorbitol MacConkey Agar

d

Lauryl Tryptose Broth (LTB)

2

In the process of microbial characterization, the laboratory analyst observed a distinct purple coloration in the results of the conducted gram staining. What classification of bacteria can be inferred to be present in the analyzed sample

a

Gram-positive

b

Gram-negative

c

Both A and B

d

Cannot be determined

3

The following results of biochemical test characterize Staphylococcus aureus, except:

a

Catalase negative

b

Coagulase positive

c

Thermonuclease positive

d

Glucose fermentation positive

4

Which culture medium is usually used for the isolation, cultivation and enumeration of lactic acid bacteria?

a

MRS (De Man, Rogosa and Sharpe) Agar

b

Potato Dextrose Agar

c

Dichloran rose bengal chloramphenicol (DRBC) agar

d

Eosin Methylene Blue (EMB) Agar

5

Which of the following microorganisms is considered very potent, doubling as fast as 10 minutes in optimum temperature?

a

Clostridium pefrigens

b

Staphylococcus aureus

c

Escherichia coli

d

Listeria monocytogenes

6

What are the IMViC results for E. coli?

a

+-+-

b

++--

c

+---

d

--++

7

The following type of media can be used in the identification of Salmonella spp. except

a

Blood Agar

b

Xylose lysine deoxycholate agar

c

Hektoen enteric agar

d

Bismuth sulfite agar

8

The following are examples of molecular methods for the detection and enumeration of foodborne microorganisms, except:

a

ELISA

b

PCR

c

DNA Sequencing

d

Rea-time PCR

9

Which of the following are not true about spoilage microorganisms? I. All spoilage microorganisms are also pathogenic microorganisms II. Spoilage microorganisms can contaminate the food only after processing III. Spoilage microorganisms produce substances that alter the sensory quality of foods IV. Spoilage microorganisms cannot mediate deterioration when the cells are killed byfood processing methods

a

I, II, IV only

b

I, II only

c

I, II, III only

d

II only

10

The following observations indicates a positive presence of E.coli, except:

a

produce green colonies, with or without metallic sheen

b

give IMViC patterns of ++--(biotype1) or -+-- (biotype2)

c

appear as Gram-negative nonsporeforming rods

d

ferment lactose with gas production

11

Microbial examination for drinking water shall provide the numbers/presence of the following microorganisms, except:

a

None of the above

b

Total coliform

c

Heterotrophic bacteria

d

E.coli/Thermotolerant coliform

12

In BAM, what is the most probable number (MPN) technique commonly used for?

a

Estimating low concentrations of coliforms and Eschirichia coli

b

Enumeration of viruses in food samples

c

Confirming toxin production in foodborne pathogens

d

Measuring pH in fermented foods

13

How many plate(s) should be used in reporting aerobic plate counts?

a

2

b

1

c

4

d

3

14

How many plates of Baird-Parker Agar are use for the isolation and enumeration of Staphylococcus aureus

a

3

b

2

c

1

d

4

15

In biochemical testing, Salmonella typically gives positive results in all of the following except

a

Phenol red lactose test

b

Polyvalent flagellar test

c

Polyvalent somatic test

d

Methyl red test

16

Escherichia coli is commonly found in the intestinal tract and can grow whether oxygen is present or absent, shifting its metabolism depending on environmental conditions. In terms of its oxygen requirement, what type of bacterium is E. coli?

a

Facultative anaerobe

b

Obligate anaerobe

c

Obligate aerobe

d

Microaerophile

17

This technique for plating is frequently employed to enumerated microorganisms in a heterogenous sample, which is introduced into molten agar medium before it solidifies

a

Pour plate technique

b

Spread plate technique

c

Streak plate technique

d

Enumeration method

18

What is the required number of sample units selected from lot of pasteurized milk that must be examined for Listeria monocytogenes?

a

5

b

6

c

10

d

30

19

Typical characteristics of S. aureus and S. epidermidis are the following except for:

a

Bothe are positive on coagulase production

b

Both are positive on catalase activity

c

Bothe are positive on lysostaphin sensitivity

d

Both are positive on anaerobic utilization of glucose

20

Salmonella shows positive result in the following biochemical and seriological reactions except on:

a

Growth in KCN broth

b

Polyvalent flagellar test

c

Methyl red test

d

Purple color in lysine decarboxylase broth

Feedback

8

Molecular methods PCR DNA Sequencing Real-time PCR

13

In APC it only use duplicate

You can integrate the game into an LMS compatible with LTI 1.1 or LTI 1.3 such as Canvas, Moodle, or Blackboard. This way, the scores will be automatically saved into the platform’s gradebook.

Food Micro

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Food MicroOnline version

by Consistency

What is the typical culture medium used in the MPN method for detecting coliforms in food and water?

In the process of microbial characterization, the laboratory analyst observed a distinct purple coloration in the results of the conducted gram staining. What classification of bacteria can be inferred to be present in the analyzed sample

The following results of biochemical test characterize Staphylococcus aureus, except:

Which culture medium is usually used for the isolation, cultivation and enumeration of lactic acid bacteria?

Which of the following microorganisms is considered very potent, doubling as fast as 10 minutes in optimum temperature?

What are the IMViC results for E. coli?

The following type of media can be used in the identification of Salmonella spp. except

The following are examples of molecular methods for the detection and enumeration of foodborne microorganisms, except:

The following observations indicates a positive presence of E.coli, except:

Microbial examination for drinking water shall provide the numbers/presence of the following microorganisms, except:

In BAM, what is the most probable number (MPN) technique commonly used for?

How many plate(s) should be used in reporting aerobic plate counts?

How many plates of Baird-Parker Agar are use for the isolation and enumeration of Staphylococcus aureus

In biochemical testing, Salmonella typically gives positive results in all of the following except

Escherichia coli is commonly found in the intestinal tract and can grow whether oxygen is present or absent, shifting its metabolism depending on environmental conditions. In terms of its oxygen requirement, what type of bacterium is E. coli?

This technique for plating is frequently employed to enumerated microorganisms in a heterogenous sample, which is introduced into molten agar medium before it solidifies

What is the required number of sample units selected from lot of pasteurized milk that must be examined for Listeria monocytogenes?

Typical characteristics of S. aureus and S. epidermidis are the following except for:

Salmonella shows positive result in the following biochemical and seriological reactions except on:

Feedback

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