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Cuestionario diagnóstico: Técnicas moleculares

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Cuestionario diagnóstico: Técnicas moleculares

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Conceptos clave de biología molecular

Created by

Edith Salazar Villa
Edith Salazar Villa
Mexico

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Cuestionario diagnóstico: Técnicas moleculares
 

Cuestionario diagnóstico: Técnicas molecularesOnline version

Conceptos clave de biología molecular

by Edith Salazar Villa
1

¿Qué caracteriza al ADN frente al ARN en términos de estructura y función?

2

¿Qué tipos de PCR se usan para cuantificar y para detectar transcriptos?

3

¿Qué se busca al analizar variación genética en poblaciones?

4

¿Cuál es un concepto clave del dogma central de la biología molecular?

5

¿Qué característica distingue al ADN del ARN?

6

¿Qué enzima sintetiza ADN en la dirección 5'→3'?

7

¿Qué técnica permite separar fragmentos de ADN por tamaño?

8

¿Qué técnica se utiliza para convertir RNA en cDNA antes de PCR?

9

¿Qué método es característico para diagnóstico molecular isoter...

10

¿Qué describe mejor la variación genética como SNP?

Explicación

El ADN es típicamente doble hélice y almacena información; el ARN es una molécula de una sola hebra que participa en la expresión génica.

La qPCR cuantifica en tiempo real; la RT-PCR convierte RNA en cDNA antes de la PCR.

Se analizan frecuencias de variantes genéticas (SNP, INDEL) para entender diversidad.

Dogma central: ADN → ARNm → proteína; ARN guía la síntesis proteica.

El ARN usa ribosa y uracilo; el ADN usa desoxirribosa y timina.

La ADN polimerasa añade nucleótidos en dirección 5' a 3'.

La electroforesis separa fragmentos según tamaño en geles.

RT-PCR usa transcripción inversa para generar cDNA a partir de RNA.

La LAMP es una técnica isoterma para amplificación rápida; útil en diagnóstico.

SNP es una variación de un solo nucleótido en un sitio genético.

 
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