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Autoevaluación TP Nº3

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Autoevaluación TP Nº3

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(1)
Autoevaluación TP Nº 3 - Transformación de E. coli con el método de CaCl2

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About Quiz
biología molecular
transformacion
bioquímica
66 times made

Created by

Cecilia Di Capua
Cecilia Di Capua
Argentina

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Autoevaluación TP Nº3Online version

Autoevaluación TP Nº 3 - Transformación de E. coli con el método de CaCl2

by Cecilia Di Capua
1

De las siguientes aseveraciones, es INCORRECTO afirmar que:

Escoge una o varias respuestas

2

La selección de las células que se han transformado con el plásmido pET-MBP-GFP se realiza observando el crecimiento en placas que contienen

3

Después de realizado el choque térmico, las células se incuban 5 minutos en hielo:

4

El choque térmico:

5

El plásmido pET-MBP-GFP utilizado para transformar, lleva entre otros genes:

6

En ausencia de IPTG en la placa de cultivo las colonias bacterianas expuestas a la luz ultravioleta son de color:

7

Después de realizado el choque térmico e incubar 5 minutos en hielo, las células se incuban 30 minutos a 37 ºC con medio de cultivo para:

Explicación

Solo las células que hayan incorporado el plásmido pET-MBP18 o pET-MBP18-GFP van a crecer en medio con Amp

El marcador de selección en nuestro ensayo es el antibiótico Ampicilina

El Plásmido pET-MBP18GFP expresa la proteína fusionada GFP-MBP y la proteína que aporta resistencia a Amp codificada por el gen bla

Para que se exprese la GFP (que fluoresce al recibir luz UV) es necesario adicionar IPTG. En ausencia del inductor las colonias son blancas.

 
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